A QUANTITATIVE-DETERMINATION OF PHOTOCHEMICAL AND NON-PHOTOCHEMICAL QUENCHING DURING THE SLOW PHASE OF THE CHLOROPHYLL FLUORESCENCE INDUCTION CURVE OF BEAN-LEAVES

A QUANTITATIVE-DETERMINATION OF PHOTOCHEMICAL AND NON-PHOTOCHEMICAL QUENCHING DURING THE SLOW PHASE OF THE CHLOROPHYLL FLUORESCENCE INDUCTION CURVE OF BEAN-LEAVES
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DOI:
10.1016/0005-2728(84)90166-x
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发表时间:
1984-01-01
期刊:
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子:
--
通讯作者:
BAKER, NR
BAKER, NR
中科院分区:
其他
文献类型:
--
作者:
BRADBURY, M;BAKER, NR

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在叶绿素荧光发射从P最大值缓慢下降到T稳定值的过程中,对普通小麦叶片PS Ⅱ电子还原-氧化态的变化进行了估算。光化学和非光化学过程的荧光猝灭的相对贡献,从这些数据确定。在100 μ mol·cm-1的低光子通量密度下,m-2.cntdot. s-1,非光化学猝灭是主要贡献者的荧光下降从P到T,虽然大的电荷被观察到在光化学猝灭后立即P.在增加光强度10倍,光化学过程的贡献荧光猝灭显着减少,几乎所有的P到T荧光下降可归因于非光化学猝灭的变化。本文还讨论了引起叶片内非光化学猝灭变化的可能因素。
Estimations of the changes in the reduction-oxidation state of PS II electron in P. vulgaris leaves were made during the slow decline in chlorophyll fluorescence emission from the maximal level at P to the steady-state at T. The relative contributions of photochemical and non-photochemical processes to the fluorescence quenching were determined from these data. At a low photon flux density of 100 .mu.mol .cntdot. m-2 .cntdot. s-1, non-photochemical quenching was the major contributor to the fluorescence decline from P to T, although large charges were observed in photochemical quenching immediately after P. On increasing the light intensity 10-fold, the contribution of photochemical processes to fluorescence quenching was markedly diminished, with nearly all the P-to-T fluorescence decline being attributable to changes in non-photochemical quenching. The possible factors responisible for changes in non-photochemical quenching within the leaves are discussed.