Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis
Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis
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DOI：
10.1093/glycob/cwg017
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发表时间：
2003-03-01
期刊：
影响因子：
4.3
通讯作者：
Bowles, DJ
中科院分区：
文献类型：
--
作者：
Lim, EK;Baldauf, S;Bowles, DJ
拟南芥基因组的完整序列使得多基因家族的明确表征和它们的系统发育关系的分析成为可能。使用先前定义的糖基转移酶，使用小分子量受体的共识序列，107个基因序列被确定在拟南芥基因组中，并用于构建系统发育树。筛选重组蛋白的体外催化活性，揭示了酶对生理上重要的底物，包括激素和次级代谢产物的活性。本研究的目的是利用整个家族的系统发育关系来探索底物识别和葡萄糖基化的区域选择性的进化。羟基香豆素已被用作分析的模型底物，其中90个序列已被测定，48个序列显示识别这些化合物。该研究揭示了多基因家族14个系统发育组中的6个的活性，表明底物识别的基本特征在实质性的进化时期被保留。
The complete sequence of the Arabidopsis genome enables definitive characterization of multigene families and analysis of their phylogenetic relationships. Using a consensus sequence previously defined for glycosyltransferases that use small-molecular-weight acceptors, 107 gene sequences were identified in the Arabidopsis genome and used to construct a phylogenetic tree. Screening recombinant proteins for their catalytic activities in vitro has revealed enzymes active toward physiologically important substrates, including hormones and secondary metabolites. The aim of this study has been to use the phylogenetic relationships across the entire family to explore the evolution of substrate recognition and regioselectivity of glucosylation. Hydroxycoumarins have been used as the model substrates for the analysis in which 90 sequences have been assayed and 48 sequences shown to recognize these compounds. The study has revealed activity in 6 of the 14 phylogenetic groups of the multigene family, suggesting that basic features of substrate recognition are retained across substantial evolutionary periods.