Starch modification with microbial alpha-glucanotransferase enzymes
Starch modification with microbial alpha-glucanotransferase enzymes
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DOI：
10.1016/j.carbpol.2012.01.065
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发表时间：
2013-03-01
影响因子：
11.2
通讯作者：
Leemhuis, Hans
中科院分区：
文献类型：
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作者：
van der Maarel, Marc J. E. C.;Leemhuis, Hans
淀粉是一种农业原料，用于许多食品和工业产品。它以直链淀粉和支链淀粉的形式存在于形状不同的颗粒中。淀粉降解酶被大规模用于生产甜味剂（高果糖玉米糖浆）和浓缩葡萄糖浆，作为生物乙醇和基本化学品发酵生产的底物。在过去的二十年中，α-葡聚糖转移酶（EC 2.4.1.xx），如分支酶（EC 2.4.1.18）和4-α-葡聚糖转移酶（EC 2.4.1.25），受到了相当大的关注。这些酶不像淀粉酶那样水解淀粉。相反，α-葡聚糖转移酶通过切割和重组α-1，4-和α-1，6-糖苷键来重塑直链淀粉和支链淀粉分子的部分。在这里，我们回顾了α-葡聚糖转移酶的特性，并讨论了这些酶在新的淀粉衍生物的产生中的新兴用途。0 2012爱思唯尔有限公司版权所有。
Starch is an agricultural raw material used in many food and industrial products. It is present in granules that vary in shape in the form of amylose and amylopectin. Starch-degrading enzymes are used on a large scale in the production of sweeteners (high fructose corn syrup) and concentrated glucose syrups as substrate for the fermentative production of bioethanol and basic chemicals. Over the last two decades alpha-glucanotransferases (EC 2.4.1.xx), such as branching enzyme (EC 2.4.1.18) and 4-alpha-glucanotransferase (EC 2.4.1.25), have received considerable attention. These enzymes do not hydrolyze the starch as amylases do. Instead, alpha-glucanotransferases remodel parts of the amylose and amylopectin molecules by cleaving and reforming alpha-1,4- and alpha-1,6-glycosidic bond. Here we review the properties of alpha-glucanotransferases and discuss the emerging use of these enzymes in the generation of novel starch derivatives. 0 2012 Elsevier Ltd. All rights reserved.