Clara cell 16 protein in COPD sputum: A marker of small airways damage?
Clara cell 16 protein in COPD sputum: A marker of small airways damage?
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DOI：
10.1016/j.rmed.2007.05.023
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发表时间：
2007-10-01
影响因子：
4.3
通讯作者：
Canonica, Giorgio Walter
中科院分区：
文献类型：
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作者：
Braido, Fulvio;Riccio, Anna M.;Canonica, Giorgio Walter
理由：吸烟者慢性阻塞性肺疾病（COPD）的发展及其对感染的易感性尚未完全了解。最近的证据表明，Clara细胞通过产生特定因子如Clara细胞16（CC-16）在宿主防御、免疫调节反应和气道重塑中发挥作用。这种蛋白质从来没有涉及到患者的肺功能测试，血气参数和疾病severity.Objectives：评估之间的可能相关性CC-16的表达在痰液中，通过一种新的方法学方法测量，和严重程度的中度和重度COPD患者。我们还分析了CC-16和痰细胞学人口，动脉血气和肺function.Main结果之间可能存在的相关性：我们分析了20例患者，平均年龄72.95，分类的基础上，全球倡议慢性阻塞性肺疾病指南（GOLD 2006）。处理样品进行细胞学分析，并通过Western印迹评估CC-16水平。我们发现重度COPD患者的CC-16水平低于中度COPD患者（p < 0.027）。CC-16表达与痰液细胞构成（巨噬细胞除外）、动脉血气和肺功能参数之间无统计学显著差异。CC-16与功能和细胞学参数的多元线性回归分析显示没有显着significant.Conclusions：我们发现了一个显着不同的CC-16在COPD患者的表达，根据其严重程度的阶段，定义的黄金2006年指南。考虑到CC-16在先天免疫中的特性，可以假设蛋白质表达、先天免疫系统和COPD感染性加重之间可能存在联系，但需要进一步研究。(C)2007年由Elsevier Ltd.出版
Rationale: The development of chronic obstructive pulmonary disease (COPD) in smokers and their susceptibility to infections is not fully understood. Recent evidences suggest that Clara cells play a part in host defense, immunomodulatory response and airways remodelling through the production of specific factors such as Clara cell 16 (CC-16). This protein has never been related to patients' lung function tests, blood gases parameters and diseases severity.Objectives: To evaluate a possible correlation between CC-16 expression in sputum, measured by a new methodological approach, and the degree of severity in patients with moderate and severe COPD. We also analyzed possible correlations between CC-16 and cytological sputum population, arterial blood gases and lung function.Main Findings: We analyzed 20 patients, mean age 72.95, classified on the basis of the global initiative for chronic obstructive lung disease guidelines (GOLD 2006). The samples were processed for cytological analysis and CC-16 levels were assessed by Western blot. We found lower levels of CC-16 in severe COPD compared to moderate ones (p < 0.027). No statistically significant differences were found between CC-16 expression and sputum cellularity (except for macrophages), arterial blood gases, and spirometric parameters. Multiple linear regression analysis of CC-16 versus functional and cytological parameters showed no significance.Conclusions: We found a significantly different expression of CC-16 in COPD patients, according to their stage of severity, as defined by the GOLD 2006 guidelines. Considering CC-16 properties in innate immunity, a possible Link between protein expression, innate immune system, and COPD infectious exacerbations may be hypothesized but further investigation are needed. (C) 2007 Published by Elsevier Ltd.