Human amniotic mesenchymal cells have some characteristics of cardiomyocytes
Human amniotic mesenchymal cells have some characteristics of cardiomyocytes
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DOI：
10.1097/01.tp.0000149503.92433.39
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发表时间：
2005-03-15
期刊：
影响因子：
6.2
通讯作者：
Nikaido, T
中科院分区：
文献类型：
--
作者：
Zhao, P;Ise, H;Nikaido, T
背景细胞心肌成形术（CCM）是治疗心力衰竭的主要方法，因为成年心肌细胞在缺血性损伤后不能再生，这导致心力衰竭。人们对寻找合适的新细胞来源用于CCM非常感兴趣。在这里，我们报告，人羊膜间充质细胞（hAMC），这是多能细胞来源于胎儿中胚层，可能是一个合适的细胞来源CCM。用逆转录聚合酶链反应和免疫细胞化学方法检测心肌特异性基因的表达。将hAMC与新生大鼠心脏外植体共培养，并移植到大鼠心脏的心肌梗死部位。结果。hAMC表达心脏特异性转录因子GATA 4、心脏特异性基因（如肌球蛋白轻链（MLC）-2a、MLC-2 v、cTnI和cTnT）以及心脏特异性L型钙通道（α 1c）和瞬时外向钾通道（Kv4.3）的α亚基。用碱性成纤维细胞生长因子（bFGF）或激活素A刺激后，hAMC表达Nkx2.5，这是心肌细胞和心脏特异性标记物心房利钠肽的特异性转录因子。此外，用激活素A处理后，检测到心脏特异性基因α-肌球蛋白重链。共培养实验证实，hAMC能够整合到心脏组织中并分化为心肌样细胞。将hAMC移植到大鼠心肌梗死区后，hAMC在瘢痕组织中存活至少2个月，并分化为心肌样细胞。本研究结果提示hAMC具有心肌细胞的某些特征。
Background. Cellular cardiomyoplasty (CCM) is a major method for the treatment of heart failure because adult cardiomyocytes do not regenerate after ischemic injury, which results in heart failure. There is a great deal of interest in finding suitable new cell sources for use in CCM. Here, we report that human amniotic mesenchymal cells (hAMC), which are multipotent cells derived from fetal mesoderm, may be a suitable cell source for CCM.Methods. Freshly isolated hAMC were examined to detect the expression of cardiac-specific genes by reverse-transcription polymerase chain reaction and immunocytochemistry. hAMC were cocultivated with neonatal rat heart explants and transplanted into myocardial infarcts in the rat heart.Results. hAMC expressed cardiac-specific transcription factor GATA4, cardiac-specific genes, such as myosin light chain (MLC)-2a, MLC-2v, cTnI, and cTnT, and the alpha-subunits of the cardiac-specific L-type calcium channel (alpha 1c) and the transient outward potassium channel (Kv4.3). After stimulation with basic fibroblast growth factor (bFGF) or activin A, hAMC expressed Nkx2.5, a specific transcription factor for the cardiomyocyte and cardiac-specific marker atrial natriuretic peptide. In addition, the cardiac-specific gene a-myosin heavy chain was detected after treatment with activin A. Coculture experiments confirmed that hAMC were able to both integrate into cardiac tissues and differentiate into cardiomyocyte-like cells. After transplantation into the myocardial infarcts in rat hearts, hAMC survived in the scar tissue for at least 2 months and differentiated into cardiomyocyte-like cells.Conclusion. The results of the present study suggest that hAMC possess some characteristics of cardiomyocytes.