Activation of the dynein adenosinetriphosphatase by cross-linking to microtubules.
Activation of the dynein adenosinetriphosphatase by cross-linking to microtubules.
复制标题
通过与微管交联激活动力蛋白腺苷三磷酸酶。
DOI：
10.1021/bi00443a035
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Johnson,KA
中科院分区：
文献类型：
--
作者：
Shimizu,T;Marchese-Ragona,SP;Johnson,KA
材料和方法试剂。 EDC来自Sigma。紫杉醇是国家癌症研究所（马里兰州贝塞斯达）的慷慨礼物。其他试剂为分析纯，如所述（Shimizu & Johnson，1983a，b）。通过MilliQ系统(Millipore)对水进行蒸馏和去离子。蛋白质纯化。通过先前描述的方法(Porter & Johnson，1983a)从嗜热四膜虫菌株B-255的纤毛制备动力蛋白。在本研究中，仅使用22S动力蛋白，术语“动力蛋白”指的是22S动力蛋白。
Materials and MethodsReagents. EDC was from Sigma. Taxol was a generous gift from the National Cancer Institute (Bethesda, MD). Other reagents were of analytical grade, as described (Shimizu & Johnson, 1983a, b). Water was distilled and deionized by the MilliQ system (Millipore). Protein Purification. Dynein was prepared fromcilia of Tetrahymena thermophila strain B-255 by the method described previously (Porter & Johnson, 1983a). In this study, only 22S dynein was used, and the term “dynein” will refer to 22S dynein.