Evaluation of process performance and retained sludge properties of a psychrophilic UASB reactor for treatment of iso-plophyl alcohol (2-propanol)-containing wastewater
Evaluation of process performance and retained sludge properties of a psychrophilic UASB reactor for treatment of iso-plophyl alcohol (2-propanol)-containing wastewater
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耐冷 UASB 反应器处理含异丙醇（2-丙醇）废水的工艺性能和滞留污泥特性评估
DOI：
10.1080/10934529.2018.1530334
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Syutsubo Kazuaki
中科院分区：
文献类型：
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作者：
Danshita Tsuyoshi;Miyaoka Yuma;Sumino Haruhiko;Iguchi Akinori;Yamaguchi Takashi;Syutsubo Kazuaki
在这项研究中，进行了连续进料实验与合成异丙醇（2-丙醇）的废水使用一个实验室规模的耐冷UASB反应器，以评估工艺性能和保留的污泥性质。为了顺利驯化，接种产甲烷颗粒污泥，并在含蔗糖和挥发性脂肪酸的合成废水中逐步增加异丙醇的比例，从0%到30%，60%，然后90%的COD（化学需氧量）。结果，经过4周的时间适应2-丙醇降解，COD去除率达到95%，在8.4公斤COD/m3/天的有机负荷率（OLR）。此外，保留的颗粒污泥的物理性质保持，即使当反应器供应2-丙醇丰富的废水超过200天。从使用血清瓶的批试验中，观察到2-丙醇的产甲烷降解和丙酮蓄积。通过比较，2-丙醇降解明显抑制在氯仿作为甲烷菌的特异性抑制剂的存在下。针对16 S rRNA基因的结构域古菌群落结构分析表明，在2-丙醇驯化的污泥中，甲烷细粉菌属的相对丰度增加。这些结果表明，颗粒污泥中的甲烷细颗粒菌相关物种在厌氧条件下对2-丙醇直接降解为丙酮有明显的贡献。
In this study, a continuous feeding experiment was conducted with synthetic iso-plophyl alcohol (2-propanol)-containing wastewater using a lab-scale psychrophilic UASB reactor to evaluate process performance and retained sludge properties. For smooth acclimation, methanogenic granular sludge was seeded and a proportion of 2-propanol in the synthetic wastewater containing sucrose and volatile fatty acids was increased stepwise from 0% to 30%, 60% and then 90% of COD (chemical oxygen demand). As a result, after a 4-week period for acclimation to 2-propanol degradation, a COD removal rate of 95% was achieved at an organic loading rate (OLR) of 8.4 kg COD/m3/day. Additionally, the physical properties of the retained granular sludge were maintained even when the reactor was supplied with 2-propanol-rich wastewater for more than 200 days. From the batch assays using serum bottles, methanogenic degradation of 2-propanol was observed with acetone accumulation. By comparison, 2-propanol degradation was clearly inhibited in the presence of chloroform as a specific inhibitor of methanogen. A domain archaeal community structure analysis targeting 16S rRNA genes showed the relative abundance of the genusMethanospilliumwas increased in the 2-propanol acclimated sludge. These results suggestedMethanospilliumrelated species in the granular sludge appreciably contributed to the direct degradation of 2-proapanol into acetone under an anaerobic condition.