Analysis of formalin-fixed, paraffin-embedded (FFPE) tissue via proteomic techniques and misconceptions of antigen retrieval
Analysis of formalin-fixed, paraffin-embedded (FFPE) tissue via proteomic techniques and misconceptions of antigen retrieval
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DOI：
10.2144/000114414
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发表时间：
2016-05-01
期刊：
影响因子：
2.7
通讯作者：
Padula, Matthew P.
中科院分区：
文献类型：
--
作者：
O'Rourke, Matthew B.;Padula, Matthew P.
自世纪后期出现以来，甲醛固定已成为保存临床样品组织的标准方法。甲醛固定的优点是固定的组织可以在室温下储存数十年而无需担心降解。这导致了包含数千个临床重要样本的巨大组织库的产生。在这里，我们审查技术的福尔马林固定，石蜡包埋（FFPE）组织样本的蛋白质组学分析，特别侧重于用于提取和打破甲醛交联的方法。我们还讨论了与称为“抗原修复”的技术相关的解释错误。“我们发现这个术语被错误地应用于两种不同的分子技术;因此，我们认为需要改变术语以确保准确报告实验结果。最后，我们建议需要更多的调查，以充分了解甲醛固定的过程及其随后的逆转。
Since emerging in the late 19th century, formaldehyde fixation has become a standard method for preservation of tissues from clinical samples. The advantage of formaldehyde fixation is that fixed tissues can be stored at room temperature for decades without concern for degradation. This has led to the generation of huge tissue banks containing thousands of clinically significant samples. Here we review techniques for proteomic analysis of formalin-fixed, paraffin-embedded (FFPE) tissue samples with a specific focus on the methods used to extract and break formaldehyde crosslinks. We also discuss an error-of-interpretation associated with the technique known as "antigen retrieval." We have discovered that this term has been mistakenly applied to two disparate molecular techniques; therefore, we argue that a terminology change is needed to ensure accurate reporting of experimental results. Finally, we suggest that more investigation is required to fully understand the process of formaldehyde fixation and its subsequent reversal.