T-bet expression is upregulated in active Behcet's disease
T-bet expression is upregulated in active Behcet's disease
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DOI：
10.1136/bjo.87.10.1264
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发表时间：
2003-10-01
影响因子：
4.1
通讯作者：
Kijlstra, A
中科院分区：
文献类型：
--
作者：
Li, B;Yang, P;Kijlstra, A
目的：目的：探讨白塞病（Behcet's disease）活动性葡萄膜炎患者外周血单个核细胞（PBMC）中T-bet mRNA和蛋白的表达。分别采用半定量逆转录聚合酶链反应（RT-PCR）和western blot法检测PBMC中T-bet mRNA和蛋白的表达。对PCR产物进行测序。为了确定活化对T-bet表达的影响，还评估了来自每个样品的植物血凝素（PHA）刺激的PBMC的T-bet mRNA和蛋白的表达。在活动性白塞氏病患者中可检测到62 kDa条带，但在对照组中不可检测到。白塞病患者活动性葡萄膜炎和正常对照组之间无差异被发现关于无论是T-bet mRNA或其蛋白的表达后，刺激与PHA的72 h.Conclusion：白塞病是与T-bet表达上调，这支持了一个角色的Th 1亚群的T细胞在这种疾病的发病机制。
Aim: To investigate T-bet mRNA and protein expression on peripheral blood mononuclear cells (PBMC) in patients with Behcet's disease with active uveitis.Methods: Blood samples were taken from 24 patients with Behcet's disease who had active uveitis and 16 healthy individuals. PBMC were subjected to analysis of T-bet mRNA and protein expression using semiquatitative reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot, respectively. The products from PCR were sequenced. In order to determine the influence of activation on T-bet expression, the phytohaemagglutinin (PHA) stimulated PBMC from each sample were also evaluated for expression of T-bet mRNA and protein.Results: A significantly increased T-bet mRNA accumulation was detected in the samples from patients with active Behcet's disease compared with that in controls. A 62 kDa band was detectable in patients with active Behcet's disease, but not in controls. No difference was found between patients with Behcet's disease who had active uveitis and normal controls concerning the expression of either T-bet mRNA or its protein after stimulation with PHA for 72 hours.Conclusion: Behcet's disease is associated with an upregulation of T-bet expression, which supports a role for the Th1 subset of T cells in the pathogenesis of this disease.