TALEN-mediated Drosophila genome editing: Protocols and applications
TALEN-mediated Drosophila genome editing: Protocols and applications
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TALEN 介导的果蝇基因组编辑：协议和应用
DOI：
10.1016/j.ymeth.2014.04.010
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发表时间：
2014-08-15
期刊：
影响因子：
4.8
通讯作者：
Jiao, Renjie
中科院分区：
文献类型：
--
作者：
Liu, Jiyong;Chen, Yixu;Jiao, Renjie
TALES(转录激活子样效应子)是一类天然转录激活子家族，最初从植物病原菌Xanthomonas spp中分离出来。故事的DNA结合基序可以重新设计，使其与特定的DNA序列结合，而不是原来的目标。将定制的故事与内切酶FOK I融合在一起，可以产生针对特定DNA位点的人造酶进行切割，从而允许对动物和植物基因组进行基因特异性修改。此前，我们曾报道使用TALEN(转录激活子样效应子核酸酶)对果蝇的两个基因座黄色和CG9797进行高度特异和高效的修饰。在这里，我们描述了一种详细的TALEN介导的果蝇基因组修饰方案，目的是为果蝇研究社区提供一个实用的工作台指南。(C)2014 Elsevier Inc.保留所有权利。
TALEs (transcription activator-like effectors) are a family of natural transcriptional activators originally isolated from the plant pathogen of Xanthomonas spp. The DNA binding motif of TALEs can be re-designed in such way that they bind specific DNA sequences other than their original targets. Fusion of customized TALEs with an endonuclease, Fok I, generates artificial enzymes that are targeted to specific DNA sites for cutting, allowing gene specific modification of both animal and plant genomes. Previously, we reported the use of TALEN (transcription activator-like effector nuclease) for the highly specific and efficient modification of the two Drosophila loci yellow and CG9797. Here, we describe a detailed protocol for TALEN-mediated genomic modification in Drosophila, with the aim of providing a practical bench guide for the Drosophila research community. (C) 2014 Elsevier Inc. All rights reserved.