Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches
Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches
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DOI：
10.1186/1471-2121-11-5
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发表时间：
2010-01-20
期刊：
影响因子：
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通讯作者：
Alcalay, Myriam
中科院分区：
文献类型：
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作者：
Licciulli, Silvia;Luise, Chiara;Alcalay, Myriam
背景：Pirin（PIR）是一种高度保守的核蛋白，最初作为NFI/CTF 1转录/复制因子的相互作用因子分离。它是功能多样的cupin超家族的成员，其活性与不同的生物和分子过程有关，如转录调节、凋亡、应激反应和酶促过程。虽然它在这些功能中的确切作用尚未被定义，PIR的表达是已知的在几个人类malignancy.Results：我们进行了免疫组织化学分析的PIR表达的主要样品从正常的人体组织和肿瘤，并确定了一个位错的PIR细胞质中的一个子集的黑色素瘤，和细胞质PIR水平和黑色素瘤的进展之间的正相关性。随后通过基于高含量免疫荧光的方法在体外分析了PIR在黑素瘤细胞系中的定位结论：通过免疫组织化学和ImmunoCell-Array获得的体内和体外结果之间的高度一致性验证了ImmunoCell-Array技术用于快速筛选推定的生物标志物的潜力，提示PIR的细胞质定位可能代表黑色素瘤进展的特征。
Background: Pirin (PIR) is a highly conserved nuclear protein originally isolated as an interactor of NFI/CTF1 transcription/replication factor. It is a member of the functionally diverse cupin superfamily and its activity has been linked to different biological and molecular processes, such as regulation of transcription, apoptosis, stress response and enzymatic processes. Although its precise role in these functions has not yet been defined, PIR expression is known to be deregulated in several human malignancies.Results: We performed immunohistochemical analysis of PIR expression in primary samples from normal human tissues and tumors and identified a dislocation of PIR to the cytoplasm in a subset of melanomas, and a positive correlation between cytoplasmic PIR levels and melanoma progression. PIR localization was subsequently analyzed in vitro in melanoma cell lines through a high content immunofluorescence based approach (ImmunoCell-Array).Conclusions: The high consistency between in vivo and in vitro results obtained by immunohistochemistry and ImmunoCell-Array provides a validation of the potential of ImmunoCell-Array technology for the rapid screening of putative biological markers, and suggests that cytoplasmic localization of PIR may represent a characteristic of melanoma progression.