A multifunctional surface for blood contact with fibrinolytic activity, ability to promote endothelial cell adhesion and inhibit smooth muscle cell adhesion
A multifunctional surface for blood contact with fibrinolytic activity, ability to promote endothelial cell adhesion and inhibit smooth muscle cell adhesion
复制标题
具有纤溶活性的血液接触多功能表面，能够促进内皮细胞粘附并抑制平滑肌细胞粘附
DOI：
10.1039/c6tb02808j
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发表时间：
2017-01-21
影响因子：
7
通讯作者：
Brash, John L.
中科院分区：
文献类型：
--
作者：
Gu, Hao;Chen, Xianshuang;Brash, John L.
各种医疗器械都需要血液相容性材料。然而，尽管经过多年的努力，血液相容性问题，特别是防止血栓形成的能力，仍然没有得到解决。基于血管内皮（最终的血液接触表面）利用几种机制来维持血液流动性的知识，类似的多功能性应该是血液相容性生物材料的目标似乎是合理的。在本工作中，聚氨酯表面改性的聚（2-羟乙基甲基丙烯酸酯-co-6-氨基-2-（2-甲基酰氨基）-己酸-co-1-金刚烷-1-甲基丙烯酸甲酯）（聚（HEMA-co-LysMA-co-AdaMA）），称为PU-PHLA。聚（HEMA）和聚（LysMA）旨在分别提供对非特异性蛋白质吸附的抗性和溶解初始凝块的能力。肝素样部分，磺化β-环糊精固定在PU-PHLA通过主客体相互作用与聚（AdaMA）。预期该组分可抑制凝血和平滑肌细胞增殖，并促进内皮化。所得到的材料被证明具有多功能性，包括纤溶活性，抗凝活性和促进内皮细胞粘附和抑制平滑肌细胞粘附的能力。本工作为开发多功能、仿内皮生物材料用于血液接触应用提供了新的策略。
Blood compatible materials are required for a wide variety of medical devices. Despite many years of intensive effort, however, the blood compatibility problem, in particular the ability to prevent thrombosis, remains unsolved. Based on the knowledge that the vascular endothelium, the ultimate blood contacting surface, draws on several mechanisms to maintain blood fluidity, it seems reasonable that analogous multifunctionality should be the goal for blood compatible biomaterials. In the present work, a polyurethane surface was modified with the terpolymer poly(2-hydroxyethyl methacrylate-co-6amino-2-(2-methacylamido)-hexanoic acid-co-1-adamantan-1-ylmethyl methacrylate) (poly(HEMA-coLysMA-co-AdaMA)), referred to as PU-PHLA. Poly(HEMA) and poly(LysMA) were intended to provide, respectively, resistance to non-specific protein adsorption and the ability to lyse incipient clots. The heparin-like moiety, sulfonated beta-cyclodextrin was immobilized on the PU-PHLA via host-guest interactions with the poly(AdaMA). This component is expected to inhibit coagulation and smooth muscle cell proliferation and to promote endothelialization. The resulting materials were shown to have multifunctionalities including fibrinolytic activity, anticoagulant activity and the ability to promote endothelial cell adhesion and inhibit smooth muscle cell adhesion. This work provides a new strategy for the development of multifunctional, endothelial-mimicking, biomaterials for blood contacting applications.