TRANSLATION INITIATION AT ALTERNATE IN-FRAME AUG CODONS IN THE RABIES VIRUS PHOSPHOPROTEIN MESSENGER-RNA IS MEDIATED BY A RIBOSOMAL LEAKY SCANNING MECHANISM
TRANSLATION INITIATION AT ALTERNATE IN-FRAME AUG CODONS IN THE RABIES VIRUS PHOSPHOPROTEIN MESSENGER-RNA IS MEDIATED BY A RIBOSOMAL LEAKY SCANNING MECHANISM
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DOI：
10.1128/jvi.69.2.707-712.1995
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发表时间：
1995-02-01
影响因子：
5.4
通讯作者：
BLONDEL, D
中科院分区：
文献类型：
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作者：
CHENIK, M;CHEBLI, K;BLONDEL, D
狂犬病病毒的磷蛋白是由P基因最长的开放阅读框编码的297个氨基酸组成的多肽。使用针对P蛋白的单抗血清的免疫沉淀实验在感染细胞、感染野生型P蛋白的细胞和纯化的病毒(CVS株)中检测到P蛋白和至少四个额外的较短产物。通过缺失分析，这些蛋白质是从次级下游的框内AUG起始密码子翻译而来的。免疫荧光实验表明，所有这些P产物均存在于细胞质中，但从8月第3、4、5密码子开始的蛋白质主要分布在细胞核中。P基因5‘末端区域的变化(包括位点特异性突变、缺失和插入)表明，泄漏扫描机制导致了P基因在几个位点上的翻译起始。
The phosphoprotein of rabies virus is a 297 amino-acid polypeptide encoded by the longest open reading frame of the P gene. Immunoprecipitation experiments using a monoclonal antiserum directed against the P protein detected the P protein and at least four additional shorter products in infected cells, cells transfected with a plasmid encoding the wild-type P protein, and purified virus (CVS strain). By means of deletion analyses, these proteins were shown to be translated from secondary downstream in-frame AUG initiation codons. Immunofluorescence experiments indicated that all these P products were found in the cytoplasm of transfected cells; however, the proteins initiated from the third, fourth, and fifth AUG codons were found mostly in the nucleus. Changes in the 5'-terminal region of the P mRNA (including site-specific mutations, deletions, and insertions) demonstrated that a leaky scanning mechanism is responsible for translation initiation of the P gene at several sites.