SEQUENTIAL RESONANCE ASSIGNMENTS IN DNA H-1-NMR SPECTRA BY TWO-DIMENSIONAL NOE SPECTROSCOPY
SEQUENTIAL RESONANCE ASSIGNMENTS IN DNA H-1-NMR SPECTRA BY TWO-DIMENSIONAL NOE SPECTROSCOPY
复制标题
二维 NOE 光谱对 DNA H-1-NMR 谱的顺序共振归属
DOI：
10.1021/ja00347a075
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发表时间：
1983
影响因子：
15
通讯作者：
R. Kaptein
中科院分区：
文献类型：
--
作者：
R. Scheek;N. Russo;R. Boelens;R. Kaptein
图1. 360-d（TGAGCGG）和d（CCGCTCA）的等摩尔混合物的MHz 2-D NOE光谱（等高线图），双链体中5 mM，在50 mM磷酸盐、0.2 MKCl、0.02%（w/v）NaN 3中，T= 28 ℃，pH 6.5（在2 H2O中的仪表阅读）。使用（ir/2-il-ir/2-Tm-ir/2-t2）n序列，在每次采集之后具有2秒弛豫延迟和0.2秒混合时间rm。在512个f值处累积了2048个点的128个自由感应衰减，在r2（±5000 Hz）中使用正交相位检测，载波频率在频谱的低场侧。脉冲和接收器相位通过一个32步相位程序循环，该程序保持了纯幅度调制的完整性，并且只收集了真实的t ~* 数据。16高斯乘法17和零填充在两个维度中的傅立叶变换之前，并且示出了对称化的纯吸收相位谱。所画的线连接d（TGAGCGG）链中碱基质子与糖H1 '质子（1A）或糖H2'和H2”质子（1B）之间的核苷酸内（用点表示）和核苷酸间接触。在图1C中，显示了相应的H1 ′-H2 ′，H2 ″（核苷酸内）交叉峰。图1D示意性地示出了对角线上的HI'谐振位置。
Figure 1. 360-MHz 2-D NOE spectrum (contour plot) of an equimolar mixture of d (TGAGCGG) and d (CCGCTCA), 5 mM in duplex, in 50 mM phosphate, 0.2 Mkc1, 0.02%(w/v) NaN3, T= 28 C, pH 6.5 (meter reading in 2H20). A (ir/2-il-ir/2-Tm-ir/2-t2)„sequence was used, 10 with a 2-s relaxation delay after each acquisition and 0.2-s mixing time rm. 128 free induction decays of 2048 points were accumulated at 512 f¡ values, using quadrature-phase detection in r2 (±5000 Hz) with the carrier frequency at the low-field side of the spectrum. The pulses and receiver phases were cycled through a 32-step phase programthat keeps intact the pure amplitude modulation, and only real t¡ data were collected. 16 Gaussian multiplication17 and zero-filling preceded Fourier transformation in both dimensions and the symmetrized pure absorption phase spectrum is shown. The drawn lines connect intra-(indicated with a dot) and internucleotide contacts between base protons and sugar HI'protons (1A) or sugar H2'and H2" protons (IB) in the d (TGAGCGG) strand. In Figure 1C the corresponding Hl'-H2', H2"(intranucleotide) cross peaks are indicated. Figure ID shows schematically the HI'resonance positions on the diagonal.