Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High Resolution Imaging Mass Spectrometry
Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High Resolution Imaging Mass Spectrometry
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高分辨率成像质谱揭示人类视网膜和支持组织的脂质景观
DOI：
10.1101/2020.04.08.029538
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
K. Schey
中科院分区：
文献类型：
--
作者：
David M. G. Anderson;J. Messinger;N. Patterson;Emilio S. Rivera;A. Kotnala;J. Spraggins;R. Caprioli;C. Curcio;K. Schey
人类视网膜进化为促进复杂的视觉任务。它在从星光到阳光的光水平下支持视力，其支持组织和脉管系统调节用于视力的血浆递送的亲脂性必需品，包括类维生素A（维生素A衍生物）。人类视网膜特别令人感兴趣，因为它具有独特的高敏锐度和色觉解剖学特征，而这些特征也容易受到普遍存在的致盲疾病的影响。视网膜精致的细胞结构由许多水平排列的细胞类型组成，从而产生在组织学和诊断临床成像中可见的结构上不同的细胞、突触和血管层。MALDI成像质谱（IMS）技术适用于视网膜研究，现在能够提供低微米空间分辨率的图像，具有高水平的化学特异性。在这项研究中，一个多模态成像方法结合最近开发的高精度多图像配准的方法被用来定义脂质在人类视网膜组织中的层状，细胞和亚细胞水平的定位。IMS结合自体荧光和明场显微镜下的人视网膜部分在黄斑和周边区域获得的数据表明，在分布和丰度的脂质物种跨和单细胞类型内的差异。值得注意的是信号在黄斑的特定层内的定位，在光感受器和RPE的不同隔室内的定位，黄斑视网膜和非黄斑RPE之间的信号的互补性，以及通过单个双键不同的脂质可以具有显著不同的分布的证据。
The human retina evolved to facilitate complex visual tasks. It supports vision at light levels ranging from starlight to sunlight, and its supporting tissues and vasculature regulate plasma-delivered lipophilic essentials for vision, including retinoids (vitamin A derivatives). The human retina is of particular interest because of its unique anatomic specializations for high-acuity and color vision that are also vulnerable to prevalent blinding diseases. The retina’s exquisite cellular architecture is composed of numerous cell types that are aligned horizontally, giving rise to structurally distinct cell, synaptic, and vascular layers that are visible in histology and in diagnostic clinical imaging. Suitable for retinal investigations, MALDI imaging mass spectrometry (IMS) technologies are now capable of providing images at low micrometer spatial resolution with high levels of chemical specificity. In this study, a multimodal imaging approach combined with a recently developed method of high accuracy multi-image registration was used to define the localization of lipids in human retina tissue at laminar, cellular, and sub-cellular levels. Data acquired by IMS combined with autofluorescence and bright-field microscopy of human retina sections in macular and peripheral regions indicate differences in distributions and abundances of lipid species across and within single cell types. Of note is localization of signals within specific layers of macula, localization within different compartments of photoreceptors and RPE, complementarity of signals between macular retina and non-macular RPE, and evidence that lipids differing by a single double bond can have markedly different distributions.
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影响因子：
4.4
作者：
Sparrow, Janet R.;Yoon, Kee Dong;Yamamoto, Kazunori
通讯作者：
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