Preparation of macroporous methacrylate-based monoliths for chromatographic applications by the Reactive Gelation Process
Preparation of macroporous methacrylate-based monoliths for chromatographic applications by the Reactive Gelation Process
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DOI：
10.1016/j.chroma.2010.04.077
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发表时间：
2010-07-09
影响因子：
4.1
通讯作者：
Morbidelli, M.
中科院分区：
文献类型：
--
作者：
Bechtle, M.;Butte, A.;Morbidelli, M.
聚合物整体柱是一种相对较新的色谱分离介质。Marti等人提出的生产这种整体的创新方法，即反应凝胶法。[1]对于聚苯乙烯，大孔材料是应用于以甲基丙烯酸酯为基础的材料。结果表明，即使材料的性质不同，也可以通过与该聚合物的反应凝胶来创建大孔结构。除了用扫描电子显微镜和BET对材料进行分析外，还用多种色谱方法对材料的性能进行了分析。ISEC实验表明，与常规填充床相比，尺寸排斥效应要小得多。该材料的渗透率相当于含有4.13微米颗粒的填充床。柱效不随流速的增加而变化。由于材料的高效率，需要较短的柱子，从而补偿了相对较低的渗透性。该整体柱还表现出显著的疏水相互作用吸附能力，这使得它适合于层析纯化过程。(C)2010爱思唯尔B.V.保留所有权利。
Polymeric monoliths are a relatively new separation medium for chromatographic applications. The innovative approach to produce such monoliths, the Reactive Gelation Process, presented by Marti et al. [1] for polystyrene macroporous materials is applied to a methacrylate-based material. It is shown that it is possible to create a macroporous structure by Reactive Gelation also with this polymer even if the properties of the material are different. Besides the analysis of the material by SEM and BET, several chromatographic methods are used to analyze the material properties. The ISEC experiments showed a much smaller size exclusion effect than in conventional packed beds. The permeability of the material is comparable to a packed bed with 4.13 mu m particles. The column efficiency is not changing for increasing flow rates. Because of the high efficiency of the material, shorter columns are needed and therefore the comparatively low permeability is compensated. The monolith also exhibits a significant adsorption capacity for hydrophobic interaction, which makes it suitable for chromatographic purification processes. (C) 2010 Elsevier B.V. All rights reserved.