The C-elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell
The C-elegans RUNX transcription factor RNT-1/MAB-2 is required for asymmetrical cell division of the T blast cell
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DOI：
10.1016/j.ydbio.2005.08.034
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发表时间：
2005-11-15
影响因子：
2.7
通讯作者：
Kohara, Y
中科院分区：
文献类型：
--
作者：
Kagoshima, H;Sawa, H;Kohara, Y
RUNX基因编码保守的转录因子，在多种动物和人类疾病的发生发展中发挥重要作用。果蝇矮小基因是一个次级配对规则基因，调节胚胎分割。人类RUNX1，以前被称为AML1，是造血所必需的。线虫RNT-1与人类RUNX基因同源。我们发现RNT-1：：GFP在胚胎的H0-2、V1-6和T母细胞中表达，并且主要在幼虫到成体的缝隙细胞中表达。RNT-1突变体在两性T细胞不对称分裂中表现出极性的丧失，在雄性尾巴中表现出异常的射线形态。遗传和分子分析表明，RNT-1与MAB-2是等位基因。突变分析表明，RNT-1/MAB-2与WRIT信号通路共同参与调节T细胞的极性。GFP：：POP-1和TLP-1：：GFP报告基因在RNT-1/MAB-2突变体中的表达研究表明，该基因在TLP-1的上游和下游，或平行于POP-1，在控制T细胞分裂不对称的遗传级联中发挥作用。我们的研究结果表明，RNT-1/MAB-2与POP-1共同调控T细胞分裂的不对称性。(C)2005 Elsevier Inc.保留所有权利。
The RUNX genes encode conserved transcription factors, which play vital roles in the development of various animals and human diseases. Drosophila runt is a secondary pair-rule gene, which regulates embryo segmentation. Human RUNX1, previously known as AML1, is essential for hematopoiesis. C elegans rnt-1 is co-orthologous to the human RUNX genes. We found that RNT-1::GFP is expressed in the H0-2, V1-6, and T blast cells in the embryo, and predominantly in the seam cells during larval to adult stages. rnt-1 mutants exhibit a loss of polarity in the asymmetrical T cell division in hermaphrodites and abnormal ray morphology in the male tail. Genetic and molecular analysis revealed that rnt-1 is allelic to mab-2. Mutant analysis suggested that rnt-1/mab-2 is involved in regulating T blast cell polarity in cooperation with the Writ signaling pathway. Expression studies of GFP: :POP-1 and TLP-1: :GFP reporters in rnt-1/mab-2 mutants indicated that this gene functions upstream of tlp-1 and downstream, or in parallel to, pop-1 in the genetic cascade that controls asymmetry of the T cell division. All our data suggest that RNT-1/ MAB-2 functions with POP-1 to control the asymmetry of the T cell division. (C) 2005 Elsevier Inc. All rights reserved.