Phylogenetic relationships between environmental and clinical isolates of Pseudomonas fluorescens and related species deduced from 16S rRNA gene and OprF protein sequences
Phylogenetic relationships between environmental and clinical isolates of Pseudomonas fluorescens and related species deduced from 16S rRNA gene and OprF protein sequences
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DOI：
10.1078/0723-2020-00253
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发表时间：
2004-02-01
影响因子：
3.4
通讯作者：
Barray, S
中科院分区：
文献类型：
--
作者：
Bodilis, J;Calbrix, R;Barray, S
假单胞菌的主要表面蛋白OprF是一种非特异性的孔蛋白，在维持细胞形态、在低渗透压环境中生长以及与各种载体的黏附中发挥重要作用。本研究的目的是(I)对来自不同来源(水、土壤、牛奶和医院)的41个分离物的OprF蛋白和16S rRNA基因的系统发育进行比较分析；(Ii)研究与OpreE系统发育相关的生理特性。16S rRNA基因的系统发育分析将荧光假单胞菌划分为一个簇(称为荧光r-簇)，而OprF蛋白的系统发育将荧光假单胞菌划分为两个非常不同的簇(称为荧光1 o-簇和荧光2 o-簇)，这可能与该菌株的原始栖息地有关。荧光素1 o簇包含大多数非根际土壤分离物，而荧光素2 o簇包含我们所有的临床分离株和大多数根际分离株(固定在根上)。为了验证这种相关性，我们研究了两个生理特征：生长温度范围和对聚苯乙烯的非特异性黏附能力。菌株的温度范围研究没有解释两个o-簇的存在，但它确实证实了某些荧光假单胞菌在37℃下生长的能力。两个o-簇中分离株的黏附能力似乎与生态位有关。
The major surface protein of the genus Pseudomonas, OprF, is a non-specific porin that plays an important role in maintenance of cell shape, in growth in a low osmolarity environment, and in adhesion to various supports. The objectives of our study were (i) to carry out a comparative analysis of phylogenies obtained from the OprF protein and from the 16S rRNA gene in 41 isolates from various sources (water, soil, milk and the hospital) and (ii) to investigate the physiological characteristics correlated with the phylogeny of OprE We report here an important incongruence between the phylogenies of the 16S rRNA gene and the OprF protein. Phylogenetic analysis of 16S rRNA genes grouped Pseudomonas fluorescens isolates into one cluster (termed fluorescens r-cluster) whilst the phylogeny of the OprF protein divided Pseudomonas fluorescens isolates into two quite distinct clusters (termed fluorescens 1 o-cluster and fluorescens 2 o-cluster) that may be related to the original habitat of the strain. The fluorescens 1 o-cluster contained the majority of non-rhizospheric soil isolates, while the fluorescens 2 o-cluster contained all our clinical isolates and most of the rhizospheric isolates (which are fixed to the roots). In order to check this correlation, we studied two physiological characteristics: the range of growth temperature and the capacity for non-specific adhesion to polystyrene. The temperature range study for strains did not explain the existence of the two o-clusters but it did confirm the capacity of certain P. fluorescens strains to grow at 37 C. The adhesion capacities of the isolates in the two o-clusters seems to be correlated with ecological niche.