Enzymatic Profile and Antibiotic Sensitivity of Some Vibrio and Aeromonas Strains
Enzymatic Profile and Antibiotic Sensitivity of Some Vibrio and Aeromonas Strains
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一些弧菌和气单胞菌菌株的酶谱和抗生素敏感性
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
W. Bazaraa
中科院分区：
文献类型：
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作者：
M. Al;W. Bazaraa
采用API-ZYM系统和药敏试验对12株弧菌和3株气单胞菌进行了鉴定。APIZYM检测结果显示，所有菌株对α -半乳糖苷酶、p -葡糖苷酶、α -甘露糖苷酶和cc-氟葡萄糖苷酶均呈阴性。所有菌株的碱性磷酸酶、酯酶（C4）、酯酶脂肪酶（C8）、亮氨酸芳酰胺酶、酸性磷酸酶和萘酚AS-BI磷酸氢酶活性均呈阳性。对葡萄糖苷酶活性是3株气单胞菌的特征。此外，气单胞菌菌株和豆色v具有很高的n -乙酰基p -葡萄糖氨基酶活性。嗜水气单胞菌、trota气单胞菌和hollisae气单胞菌对9种抗菌药物均敏感，ClO和C30对9种抗菌药物均有活性。API-ZYM系统和抗生素敏感性谱提供了额外的信息，可以帮助鉴别和分离非常接近的弧菌和气单胞菌。
API-ZYM system and antibiotic sensitivity assay were used to characterize twelve strains of Vibrio and three strains of Aeromonas , each belonging to different species. APIZYM showed that all tested strains exhibited negative activity for a-galactosidase, P-glucuronidase, a-mannosidase and cc-flucosidase. All strains expressed positive activities for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, acid phosphatase, and naphthol AS-BI phosphohydrdase. p-glucosidase activity was a characteristic of the three Aeromonas strains. Also, Aeromonas strains as well as V. damsela gave very high N-acetyl P-glucosamidase activities. Aeromonas hydrophila, A. trota and V. hollisae were susceptible to all 9 tested antibiotics, and ClO and C30 were active against all tested strains. API-ZYM system and antibiotic susceptibility profile gave additional information that can help in identifying and separating very close species of Vibrio and Aeromonas .