Cardiovascular effects in vitro of a polysaccharide from Salvia miltiorrhiza
Cardiovascular effects in vitro of a polysaccharide from Salvia miltiorrhiza
复制标题
DOI：
10.1016/j.carbpol.2014.12.038
复制
发表时间：
2015-05-05
影响因子：
11.2
通讯作者：
Song, Yao-Ming
中科院分区：
文献类型：
--
作者：
Geng, Zhao-Hua;Huang, Lan;Song, Yao-Ming
从丹参（Salvia miltiorrhiza）根中分离得到一种多糖（SMP 1）。本研究旨在探讨SMP 1对H2 O2诱导的H9 c2细胞凋亡的保护作用。本研究表明，暴露于100 mM H2 O2的H9 c2细胞24小时引起的细胞死亡和凋亡的显着增加，但与SMP 1预处理消除H2 O2诱导的凋亡细胞死亡。此外，与SMP 1预处理显着防止线粒体破坏，细胞色素c的释放，促凋亡Bax和抗凋亡Bcl-2蛋白表达之间的比例的上升，和caspase-3激活H2 O2刺激后的H9 c2细胞。此外，经SMP 1预处理后，H2 O2诱导的PC 12细胞超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性的下降以及丙二醛（MDA）的升高均被显著逆转至正常水平。这些结果表明，SMP 1保护H9 c2细胞免受H2 O2诱导的凋亡，通过抑制线粒体功能障碍，失活caspase-3级联反应和增强抗氧化能力。(C)2015爱思唯尔有限公司版权所有。
A polysaccharide (SMP1) was isolated from the roots of Salvia miltiorrhiza. This study is designed to investigate whether SMP1 prevents H9c2 cells from hydrogen peroxide (H2O2)-induced apoptosis. The present study showed that exposure of H9c2 cells to 100 mM H2O2 for 24h caused a significant increase in cell death and apoptosis, but pretreatment with SMP1 eliminated H2O2-induced apoptotic cell death. Furthermore, pretreatment with SMP1 significantly prevented the mitochondria disruption, cytochrome c release, the rise of the ratio between proapoptotic Bax and antiapoptotic Bcl-2 protein expression, and caspase-3 activation in H9c2 cells upon H2O2 stimulation. Moreover, the decline of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities together with the elevation of malondialdehyde (MDA) in PC12 cells exposed to H2O2 were remarkably reversed to normal levels by pretreatment with SMPl. These results suggest that SMP1 protects H9c2 cells from H2O2-induced apoptosis through inhibition of mitochondrial dysfunction, inactivation of caspase-3 cascade and enhancement of antioxidant capacity. (C) 2015 Elsevier Ltd. All rights reserved.