Importance of Determination of Crystal Quality in Protein Crystals when Performing High-Resolution Structural Analysis
Importance of Determination of Crystal Quality in Protein Crystals when Performing High-Resolution Structural Analysis
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进行高分辨率结构分析时确定蛋白质晶体晶体质量的重要性
DOI：
10.1021/acs.cgd.6b00457
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发表时间：
2016
影响因子：
3.8
通讯作者：
Kenichi
中科院分区：
文献类型：
--
作者：
Koizumi;Haruhiko Suzuki;Ryo Tachibana;Masaru Tsukamoto;Katsuo Yoshizaki;Izumi Fukuyama;Seijiro Suzuki;Yoshihisa Uda;Satoshi Kojima;Kenichi
我们表明，即使在蛋白质晶体的情况下，必须考虑由衍射动力学理论解释的消光效应。利用X射线形貌术，在楔形葡萄糖异构酶晶体中，用单色同步辐射进行X射线形貌测量，在楔形葡萄糖异构酶晶体区域中，清晰地观察到了由Pendello Schlosung效应引起的半厚条纹。这表明，这种葡萄糖异构酶晶体的完美度足够高，足以产生这种动力学理论相关的效果：这肯定会导致难以收集准确的积分强度的衍射斑点的X射线结构分析。因此，重要的是确定分析中的蛋白质晶体的晶体质量是否足以获得用于X射线结构分析的蛋白质分子的精确三维结构。我们表明，X射线衍射摇摆曲线测量可以提供这种确定的线索。
We show that the extinction effect explained by the dynamical theory of diffraction must be considered even in the case of protein crystals. Equal-thickness fringes, which are attributed to the Pendellösung effect, were clearly observed in the region of a tapered glucose isomerase crystal with wedge-like edges using X-ray topography carried out with a beam of monochromatic synchrotron radiation. This indicates that the perfection of this glucose isomerase crystal is high enough to produce this dynamical theory-related effect: this surely leads to difficulty in the collection of accurate integrated intensities of diffraction spots for X-ray structural analysis. Therefore, it is important to determine whether the crystal quality of a protein crystal under analysis is adequate to obtain accurate three-dimensional structures of protein molecules for X-ray structural analyses. We show that X-ray diffraction rocking-curve measurements can provide clues for this determination.