DEVELOPMENT OF TECHNIQUES FOR GASTROJEJUNAL BYPASS SURGERY IN OBESE MICE
DEVELOPMENT OF TECHNIQUES FOR GASTROJEJUNAL BYPASS SURGERY IN OBESE MICE
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DOI：
10.1002/micr.20746
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发表时间：
2010-01-01
期刊：
影响因子：
2.1
通讯作者：
Wang, Hao
中科院分区：
文献类型：
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作者：
Lan, Zhu;Zassoko, Roman;Wang, Hao
我们以前描述了一个十二指肠空肠旁路（DJB）手术模型在健康的C57 BL/6小鼠。然而，我们的初步研究表明，同样的手术技术导致肥胖小鼠的高死亡率。在这项研究中，显着提高减肥手术后的动物存活率，从而提供一个稳定的手术模型，在肥胖小鼠的葡萄糖稳态的研究，我们已经使用了改进的技术，并开发了端侧胃空肠旁路（GJB）手术在肥胖C57 BL/6与糖耐量受损。改良方法包括：1）在幽门水平结扎远端胃; 2）以端侧方式将空肠与胃前壁连接; 3）通过端侧吻合将胆胰分泌物通过盲肢转移到远端空肠。我们发现，通过将我们原始模型中描述的近端端对端十二指肠空肠吻合术修改为这些肥胖小鼠的端侧胃空肠吻合术，我们能够显著改善本研究中的术后死亡率。我们还证明，在肥胖小鼠中进行GJB手术导致显著的体重减轻，血糖水平正常化，并预防急性胰腺炎。这种新开发的肥胖小鼠GJB手术为研究胃肠道手术治疗2型糖尿病的机制提供了独特的优势。(c)2010 Wiley-Liss，Inc.显微外科30：289-295，2010。
We have previously described a duodenojejunal bypass (DJB) surgical model in healthy C57BL/6 mice. However, our pilot study showed that the same surgical technique caused a high mortality rate in obese mice. In this study, to significantly improve animal survival rate following bariatric surgery and thereby providing a stable surgical model for the study of glucose homeostasis in obese mice, we have used modified techniques and developed the end-to-side gastrojejunal bypass (GJB) surgery in obese C57BL/6 with impaired glucose tolerance. The modification consisted of using the distal part of the jejunum for biliopancreatic diversion including: 1) ligation of the distal stomach at the level of the pylorus; 2) connection the jejunum to the anterior wall of stomach in an end-to-side fashion; and 3) diverting the biliopancreatic secretions through the blind limb into the distal jejunum through an end-to-side anastomosis. We found that by modifying the proximal end-to-end duodenojejunal anastomosis, described in our original model, to an end-to-side gastrojejunal anastomosis in these obese mice, we were able to significantly improve the postoperative mortality in this study. We have also demonstrated that performing the GJB surgery in obese mice resulted in significant weight loss, normalized blood glucose levels, and prevented acute pancreatitis. This newly developed GJB surgery in the obese mice offers a unique advantage to study the mechanisms of gastrointestinal surgery as treatment for type 2 diabetes. (c) 2010 Wiley-Liss, Inc. Microsurgery 30:289-295, 2010.