Enhanced chondrogenic responses of articular chondrocytes onto porous silk fibroin scaffolds treated with microwave-induced argon plasma
Enhanced chondrogenic responses of articular chondrocytes onto porous silk fibroin scaffolds treated with microwave-induced argon plasma
复制标题
DOI：
10.1016/j.surfcoat.2008.06.154
复制
发表时间：
2008-08-30
影响因子：
5.4
通讯作者：
Rah, Dong Kyun
中科院分区：
文献类型：
--
作者：
Baek, Hyun Sook;Park, Young Hwan;Rah, Dong Kyun
丝素蛋白（SF）作为一种天然存在的可降解纤维蛋白，具有独特的力学性能、良好的生物相容性和可加工性，在骨组织工程中显示出强大的潜力。近年来的研究主要集中在纳米纤维SF（NSF）作为一种新型的软骨形成支架，因为它的结构非常类似于天然细胞外基质（ECM）的胶原纤维结构。然而，利用微波诱导氩等离子体对NSF支架进行表面改性的研究较少。本研究基于这样的假设，即等离子体处理三维多孔静电纺丝NSF支架将促进软骨细胞的细胞生长、成软骨性和新的软骨特异性ECM形成。结果发现，等离子体处理可以诱导静电纺丝NSF支架表面的本质改性。人关节软骨细胞在表面修饰的NSF支架上的附着和增殖显著增加，伴随着糖胺聚糖合成的增加。这些结果表明，微波诱导等离子体处理的多孔NSF支架可能是有效的，以提高软骨细胞的细胞行为和软骨分化，并进一步潜在地用于软骨组织工程。(c)2008 Elsevier B.V.保留所有权利。
Silk fibroin (SF) as a naturally occurring degradable fibrous protein with unique mechanical properties, excellent biocompatibility and processability has demonstrated strong potential for skeletal tissue engineering. Recent Studies has mostly focused on nanofibrous SF (NSF) as a novel chondrogenic scaffold since its structure is very similar to collagen fibrous Structure of natural extracellular matrix (ECM). However, less attention has been paid to the surface modification of NSF scaffolds by microwave-induced argon plasma. The present study was based on the hypothesis that plasma treatment to 3-D porous electrospun NSF scaffolds would improve cell growth, chondrogenicity and new cartilage-specific ECM formation Of chondrocytes. It was found that plasma treatment could induce an essential modification of the surface of electrospun NSF scaffolds. The attachment and proliferation of human articular chondrocytes onto the surface-modified NSF scaffolds were significantly increased with a concomitant increase in the glycosaminoglycan synthesis. These results Suggest that porous NSF scaffolds treated with microwave-induced plasma may be effective for enhancing the cellular behaviors and chondrogenic differentiation of chondrocytes and further be potentially used to cartilage tissue engineering. (c) 2008 Elsevier B.V. All rights reserved.