Clinical virology of Ebola hemorrhagic fever (EHF): Virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Democratic Republic of the Congo, 1995
Clinical virology of Ebola hemorrhagic fever (EHF): Virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Democratic Republic of the Congo, 1995
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DOI：
10.1086/514321
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发表时间：
1999-02-01
影响因子：
6.4
通讯作者：
Peters, CJ
中科院分区：
文献类型：
--
作者：
Ksiazek, TG;Rollin, PE;Peters, CJ
对1995年埃博拉出血热(EHF)在Kikwit总医院治疗的患者进行了病毒抗原、免疫球蛋白G和免疫球蛋白M抗体以及传染性病毒的检测。几乎所有患者在急性期都能检测到病毒抗原，而在死亡前并不总是能检测到抗体。病毒也是在患者发烧期间从他们身上分离出来的，但试图通过标准的空斑分析来量化Vero E6细胞中的病毒通常是不成功的。Ig G和Ig M抗体在发病后(8-10天)几乎同时出现，但在存活的恢复期患者中，Ig M持续的时间要短得多。对于疑似急性流行性出血热患者，检测埃博拉病毒抗原或病毒分离似乎是最可靠的诊断手段，因为这种往往致命的疾病(80%的死亡率)患者在死前可能不会产生可检测到的抗体。
Ebola hemorrhagic fever (EHF) patients treated at Kikwit General Hospital during the 1995 outbreak were tested for viral antigen, IgG and IgM antibody, and infectious virus. Viral antigen could be detected in virtually all patients during the acute phase of illness, while antibody was not always detectable before death. Virus was also isolated from patients during the course of their febrile illness, but attempts to quantify virus in Vero E6 cells by standard plaque assay were often unsuccessful. IgG and IgM antibody appeared at approximately the same time after disease onset (8-10 days), but IgM persisted for a much shorter period among the surviving convalescent patients. IgG antibody was detectable in surviving patients through about 2 years after onset, the latest time that samples were obtained, Detection of Ebola virus antigens or virus isolation appears to be the most reliable means of diagnosis for patients with suspected acute EHF, since patients with this often-fatal disease (80% mortality) may not develop detectable antibodies before death.