Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low-Molecular-Weight Analytes
Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low-Molecular-Weight Analytes
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DOI：
10.1002/macp.201400260
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发表时间：
2014-12
影响因子：
2.5
通讯作者：
Nityanand Sharma;Christiane Petri;U. Jonas;Monika Bach;G. Tovar;K. Mrkvová;M. Vala;J. Homola;W. Knoll;J. Dostálek
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文献类型：
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作者：
Nityanand Sharma;Christiane Petri;U. Jonas;Monika Bach;G. Tovar;K. Mrkvová;M. Vala;J. Homola;W. Knoll;J. Dostálek
据报道，负载分子印迹聚合物纳米颗粒（nanoMIP）的3D水凝胶聚合物网络的新型复合层结构可用于低分子量化合物的直接光学检测。该复合材料层附着在表面等离子体共振（SPR）传感器的金属表面上，同时作为光波导和大容量结合基质用于印迹目标分析物。光波导光谱（OWS）用作无标记读出方法，允许直接测量与基质内的分子结合事件相关的折射率变化。这种方法是通过使用可光交联的聚（N-异丙基丙烯酰胺）基水凝胶和聚[（乙二醇二甲基丙烯酸酯）-（甲基丙烯酸）]纳米颗粒来实现的，所述纳米颗粒用l-Boc-苯丙氨酸-苯胺（l-BFA，分子量353 g mol−1）印迹。使用特定靶标和其他结构相似的参比化合物进行的滴定实验显示出良好的专属性，并且靶标1-BFA的检测限低至2 × 10− 6 m。
New composite layer architecture of 3D hydrogel polymer network that is loaded with molecularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low‐molecular‐weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding‐matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a label‐free readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photo‐crosslinkable poly(N‐isopropylacrylamide)‐based hydrogel and poly[(ethylene glycol dimethylacrylate)‐(methacrylic acid)] nanoparticles that are imprinted withl‐Boc‐phenylalanine‐anilide (l‐BFA, molecular weight 353 g mol−1). Titration experiments with the specific target and other structurally similar reference compounds show good specificity and limit of detection for targetl‐BFA as low as 2 × 10−6m.