The Swi/Snf chromatin remodeling complex is required for ribosomal DNA and telomeric silencing in Saccharomyces cerevisiae
The Swi/Snf chromatin remodeling complex is required for ribosomal DNA and telomeric silencing in Saccharomyces cerevisiae
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DOI：
10.1128/mcb.24.18.8227-8235.2004
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发表时间：
2004-09-01
影响因子：
5.3
通讯作者：
Winston, F
中科院分区：
文献类型：
--
作者：
Dror, V;Winston, F
Swi/Snf染色质重塑复合体先前已被证明是酿酒酵母中一部分基因的转录激活和抑制所必需的。在这项工作中，我们证明Swi/Snf也是抑制核糖体DNA （rDNA）位点中RNA聚合酶ii依赖性转录（rDNA沉默）所必需的。这种抑制似乎独立于Sir2和Set1，这两个已知的rDNA沉默所需的因子。与许多其他rDNA沉默突变体的rDNA重组水平升高相比，snf2Delta突变体的rDNA重组水平显著降低。其他研究表明，端粒附近基因的沉默也需要Swi/Snf，而对HML或HMR的沉默没有可检测到的影响。
The Swi/Snf chromatin remodeling complex has been previously demonstrated to be required for transcriptional activation and repression of a subset of genes in Saccharomyces cerevisiae. In this work we demonstrate that Swi/Snf is also required for repression of RNA polymerase II-dependent transcription in the ribosomal DNA (rDNA) locus (rDNA silencing). This repression appears to be independent of both Sir2 and Set1, two factors known to be required for rDNA silencing. In contrast to many other rDNA silencing mutants that have elevated levels of rDNA recombination, snf2Delta mutants have a significantly decreased level of rDNA recombination. Additional studies have demonstrated that Swi/Snf is also required for silencing of genes near telomeres while having no detectable effect on silencing of HML or HMR.