Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content
Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content
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DOI：
10.1101/gr.6.7.633
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发表时间：
1996-07-01
期刊：
影响因子：
7
通讯作者：
Weissman, SM
中科院分区：
文献类型：
--
作者：
Baskaran, N;Kandpal, RP;Weissman, SM
描述了使用两种酶方法（Klentaql和Pfu DNA聚合酶）和DMSO和甜菜碱的组合来均匀扩增GC含量不同的DNA模板的混合物的PCR方法。应用该方法从脆性X区域扩增CGG重复区域。
A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaql and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.