Small temperature variations alter edaravone-induced neuroprotection of cortical cultures exposed to prolonged hypoxic episodes
Small temperature variations alter edaravone-induced neuroprotection of cortical cultures exposed to prolonged hypoxic episodes
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DOI：
10.1093/bja/aep320
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发表时间：
2010-01-01
影响因子：
9.8
通讯作者：
Mashimo, T.
中科院分区：
文献类型：
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作者：
Shibuta, S.;Varathan, S.;Mashimo, T.
依达拉奉是一种自由基清除剂，已被证明在体内和体外具有神经保护作用。然而，小的温度变化对其神经保护作用的影响仍然是未知的，我们研究了不同浓度的依达拉奉在三种条件下（轻度低温（32 ℃），正常体温（37 ℃）和轻度高温（39 ℃））暴露于长期缺氧（24 h）的皮层培养物中所赋予的神经保护程度。采用缺氧前后拍摄的显微照片评价了E16 Wistar大鼠（SR）皮质神经元的存活情况，常温下缺氧神经元的平均存活率为14.7%（sem 1.8）%。添加50 μ M依达拉奉显著提高了平均存活率至40.5（4.7%）。在较高剂量的依达拉奉（5 μ M）下观察到这种改善
Edaravone, a free radical scavenger, has been shown to be neuroprotective in vivo and in vitro. However, the impact of small temperature variations on its neuroprotective actions remains unknown.We examined the degree of neuroprotection conferred by various concentrations of edaravone on cortical cultures exposed to prolonged hypoxia (24 h) under three conditions: mild hypothermia (32 degrees C), normothermia (37 degrees C), and mild hyperthermia (39 degrees C). The survival of cortical neurones from E16 Wistar rats (SR) was evaluated using photomicrographs taken before and after exposure to hypoxia.The mean survival of neurones exposed to hypoxia at normothermia was 14.7 (sem 1.8)%. The addition of 50 mu M edaravone significantly improved the mean survival to 40.5 (4.7)%. This improvement was noted at higher doses of edaravone (5 mu M