Phosphoinositide 5-Phosphate and Phosphoinositide 4-Phosphate Trigger Distinct Specific Responses of Arabidopsis Genes
Phosphoinositide 5-Phosphate and Phosphoinositide 4-Phosphate Trigger Distinct Specific Responses of Arabidopsis Genes
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DOI：
10.4161/psb.1.3.2997
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Avramova, Zoya
中科院分区：
文献类型：
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作者：
Alvarez-Venegas, Raul;Xia, Yuannan;Avramova, Zoya
磷酸肌醇磷酸盐 (PtdInsP) 是细胞脂质库的重要组成部分，可在多种细胞过程中充当信使。然而，缺乏下游靶标的信息阻碍了我们对脂质信号传导影响基因活性的潜力的理解。我们的目标是鉴定在两种异构单磷酸脂质信使（磷酸肌醇 5-磷酸，PtdIns(5) P 和磷酸肌醇 4-磷酸，PtdIns(4) P）存在时改变表达的基因，并确定这两种脂质是否影响不同或重叠的基因集。我们的结果表明，PtdIns(5) P 和 PtdIns(4) P 影响共享基因家族内的基因，但每个信使影响同一家族内不同成员的表达。这些结果表明 PtdIns(5) P 和 PtdIns(4) P 参与不同的途径，最终可能控制基因表达。这些路径可能有交汇点，但也可能抵消彼此的影响。 PtdIns(5) P 刺激基因的很大一部分（大约 40%）属于不同的壁修饰基因家族。细胞壁修饰活性被认为是影响细胞延伸和植物生长的因素。 PtdIns(5) P 浓度升高会影响茎生长，其效果与 PtdIns(4) P 引发的效果不同。这些数据可以在全植物/全基因组水平上深入了解植物对两种相关 PtdInsP 的反应，并证明 PtdIns(5) P 和 PtdIns(4) P 涉及机制是不同的、选择性的和特异性的。
Phosphoinositide phosphates, PtdInsP, are important components of the cell lipid pool that can function as messengers in diverse cellular processes. Lack of information on downstream targets, however, has impeded our understanding of the potential of lipid-signaling to influence gene activity. Our goals here were to identify genes that altered expression in the presence of two isomeric monophosphate lipid messengers (Phosphoinositide 5-Phosphate, PtdIns(5) P, and Phosphoinositide 4-Phosphate, PtdIns(4) P) and to establish whether the two lipids influence distinct or overlapping gene-sets. Our results indicated that PtdIns(5) P and PtdIns(4) P affected genes within shared gene-families but that each messenger influenced the expression of different members within the same family. These results suggested that PtdIns(5) P and PtdIns(4) P participate in separate pathways that, ultimately, may control gene expression. The pathways may have points of convergence but may also counteract each other's effects. A significant fraction (similar to 40%) of the PtdIns(5) P-stimulated genes belong to various families of wall-modifying genes. Wall-modifying activities are recognized as factors affecting cell extension and plant growth. Elevated PtdIns(5) P concentration influenced stem growth and the effects were different from those triggered by PtdIns(4) P. The data allow insights into plants' response to two related PtdInsP at whole-plant/genome-wide levels and demonstrate that PtdIns(5) P-and PtdIns(4) P-involving mechanisms are distinct, selective and specific.