DNA dendrimers localize MyoD mRNA in presomitic tissues of the chick embryo
DNA dendrimers localize MyoD mRNA in presomitic tissues of the chick embryo
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DOI：
10.1083/jcb.149.4.825
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发表时间：
2000-05-15
影响因子：
7.8
通讯作者：
George-Weinstein, M
中科院分区：
文献类型：
--
作者：
Gerhart, J;Baytion, M;George-Weinstein, M
MyoD的表达被认为是诱导体节在周围组织释放的因素，然而，逆转录-PCR和细胞培养分析表明，肌原细胞存在于胚胎体节形成之前。荧光标记的DNA树状聚合物用于在体内鉴定前体组织中的MyoD表达细胞。MyoD阳性细胞的亚群被发现在节段板，上胚层，中胚层，和下胚层。产蛋后，两层胚胎的上胚层含有类似于20个MyoD阳性细胞。这些结果表明，树枝状聚合物是精确和灵敏的试剂，用于定位组织切片和整个胚胎中低水平的mRNA，并且在原肠胚形成开始之前，胚胎中存在具有生肌潜能的细胞。
MyoD expression is thought to be induced in somites in response to factors released by surrounding tissues; however, reverse transcription-PCR and cell culture analyses indicate that myogenic cells are present in the embryo before somite formation. Fluorescently labeled DNA dendrimers were used to identify MyoD expressing cells in presomitic tissues in vivo. Subpopulations of MyoD positive cells were found in the segmental plate, epiblast, mesoderm, and hypoblast. Directly after laying, the epiblast of the two layered embryo contained similar to 20 MyoD positive cells. These results demonstrate that dendrimers are precise and sensitive reagents for localizing low levels of mRNA in tissue sections and whole embryos, and that cells with myogenic potential are present in the embryo before the initiation of gastrulation.