Effect of cigarette smoking on cough reflex induced by TRPV1 and TRPA1 stimulations
Effect of cigarette smoking on cough reflex induced by TRPV1 and TRPA1 stimulations
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DOI：
10.1016/j.rmed.2011.12.007
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发表时间：
2012-03-01
影响因子：
4.3
通讯作者：
Kohzuki, Masahiro
中科院分区：
文献类型：
--
作者：
Kanezaki, Masashi;Ebihara, Satoru;Kohzuki, Masahiro
背景：最近的研究表明，TRPA1拮抗剂可以抑制香烟引起的神经源性炎症，而TRPV1拮抗剂则不能。由于已知咳嗽反射敏感性会受到吸烟状态的影响，我们研究了吸烟对健康男性TRPA1-和trpv1诱导的咳嗽和急咳的影响。方法：采用公开招聘的方式招募健康不吸烟者26例和健康现吸烟者30例。通过吸入辣椒素（一种TRPV1激动剂）和肉桂醛（一种TRPA1激动剂）来评估咳嗽反射阈值和咳嗽冲动。咳嗽反射阈值定义为辣椒素和肉桂醛的最低浓度，分别引起两次或更多次咳嗽（C-2）和五次或更多次咳嗽（C-5）。采用改良Borg评分法评估咳嗽冲动。结果：在辣椒素引起的咳嗽中，当前吸烟者的咳嗽反射阈值C-2和C-5表达明显高于未吸烟者（p < 0.01和p < 0.001）。当前吸烟者的促咳对数斜率显著低于从不吸烟者（p < 0.001）。不吸烟者和当前吸烟者之间的咳嗽冲动阈值没有显著差异。在肉桂醛诱导的咳嗽中，从不吸烟者和现在吸烟者的C-2和C-5咳嗽反射阈值无显著差异，从不吸烟者和现在吸烟者的促咳对数斜率也无显著差异。不吸烟者和当前吸烟者之间的咳嗽冲动阈值没有显著差异。结论：吸烟对TRPV1和TRPA1刺激的咳嗽反应有不同的影响。(C) 2011 Elsevier Ltd.版权所有。
Background: Recent studies have shown that neurogenic inflammation induced by cigarette smoke is inhibited by TRPA1 antagonist, but not by TRPV1 antagonist. Since cough reflex sensitivity is known to be modified by smoking status, we investigated the effects of cigarette smoking on TRPA1- and TRPV1-induced cough and urge-to-cough in healthy males.Methods: Twenty-six healthy never-smokers and 30 healthy current smokers were recruited via public postings. Cough reflex thresholds and urge-to-cough were evaluated by inhalation of capsaicin, a TRPV1 agonist, and cinnamaldehyde, a TRPA1 agonist. The cough reflex thresholds were defined as the lowest concentrations of capsaicin and cinnamaldehyde that elicited two or more coughs (C-2) and five or more coughs (C-5), respectively. The urge-to-cough was evaluated using the modified Borg scale.Results: In capsaicin-induced cough, the cough reflex thresholds, as expressed by C-2 and C-5, in current smokers were significantly higher than those in never-smokers (p < 0.01 and p < 0.001, respectively). The urge-to-cough log log slopes in current smokers were significantly lower than those of never-smokers (p < 0.001). There were no significant differences in the thresholds of the urge-to-cough between never-smokers and current smokers. In cinnamaldehyde-induced cough, there were no significant differences in cough reflex thresholds in C-2 and C-5 between never-smokers and current smokers, nor were there any significant differences in urge-to-cough log log slope between never-smokers and current smokers. There were no significant differences in the thresholds of the urge-to-cough between never-smokers and current smokers.Conclusion: The study suggests that smoking has a differential effect on cough responses between TRPV1 and TRPA1 stimulations. (C) 2011 Elsevier Ltd. All rights reserved.