Embryogenesis plasticity and the transmission of maternal effects in Daphnia pulex.
Embryogenesis plasticity and the transmission of maternal effects in Daphnia pulex.
复制标题
溞的胚胎发生可塑性和母体效应的传递。
DOI：
10.1111/ede.12346
复制
发表时间：
2020
影响因子：
2.9
通讯作者：
Hasoon MSR
中科院分区：
文献类型：
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作者：
Hasoon MSR
了解遗传、非遗传和环境线索在发育过程中是如何整合的，对于了解生物是否以及如何对快速的环境变化做出反应可能是至关重要的。正常情况下，只有胚胎后研究才是可能的。但在这项研究中，我们开发了一种实时、高通量的共聚焦显微镜分析方法，使我们能够在个体水平上将瑞香胚胎发生与后代生活史变化联系起来。我们的分析确定了由7个发育阶段连接的8个明显的发育表型，其持续时间与特定的后代生活史轨迹的表达有关。Daphnia胚胎的发生不仅在同一环境中饲养的无性系之间存在差异，而且当母亲年龄不同或在不同的食物环境中饲养时，单个克隆内的Daphnia胚胎发生也不同。我们的结果支持这样的假设，即达夫尼亚胚胎的发生是可塑性的，可以通过母体状态或母体环境的变化来改变。除了进一步了解支持亲本效应的机制，我们的检测还可能有工业应用，如果它可以被用作快速生态毒理学预筛选，以测试污染物剂量对后代生活史的影响，传统上是通过21天的Daphniare生产测试来分析的。
Understanding how genetic, nongenetic, and environmental cues are integrated during development may be critical in understanding if, and how, organisms will respond to rapid environmental change. Normally, only post‐embryonic studies are possible. But in this study, we developed a real‐time, high‐throughput confocal microscope assay that allowed us to linkDaphniaembryogenesis to offspring life history variation at the individual level. Our assay identified eight clear developmental phenotypes linked by seven developmental stages, the duration of which were correlated with the expression of specific offspring life history traits.Daphniaembryogenesis varied not only between clones reared in the same environment, but also within a single clone when mothers were of different ages or reared in different food environments. Our results support the hypothesis thatDaphniaembryogenesis is plastic and can be altered by changes in maternal state or maternal environment. As well as furthering our understanding of the mechanisms underpinning parental effects, our assay may also have an industrial application if it can be used as a rapid ecotoxicological prescreen for testing the effect that pollutant doses have on offspring life histories traditionally assayed with a 21‐dayDaphniareproduction test.