Physiological concentrations of dopamine decrease adenosine 3',5'-monophosphate levels in cultured rat anterior pituitary cells and enriched populations of lactotrophs: evidence for a causal relationship to inhibition of prolactin release.
Physiological concentrations of dopamine decrease adenosine 3',5'-monophosphate levels in cultured rat anterior pituitary cells and enriched populations of lactotrophs: evidence for a causal relationship to inhibition of prolactin release.
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多巴胺的生理浓度降低了培养的大鼠垂体前叶细胞中的腺苷 3,5-单磷酸水平和丰富的催乳素群体：与抑制催乳素释放之间因果关系的证据。
DOI：
10.1210/endo-111-2-398
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
C. Denef
中科院分区：
文献类型：
--
作者：
L. Swennen;C. Denef
cAMP介导的多巴胺能抑制PRL释放的可能参与研究在原代培养的垂体前叶细胞从雌性大鼠和乳营养丰富的亚群。在存在磷酸二酯酶抑制剂异丁基甲基黄嘌呤（IBMX）的情况下，多巴胺（DA）在1-100 nM之间以浓度依赖性方式抑制cAMP积累。效果是显着的，在不到1分钟，最大约10分钟后。抑制PRL释放DA发生在相似的浓度和时间过程中抑制cAMP水平。DA激动剂阿扑吗啡在纳摩尔浓度下也抑制cAMP水平和PRL释放，并作为完全激动剂，而α-肾上腺素受体激动剂苯肾上腺素在1 μM下没有作用。DA受体阻断剂螺哌隆、多潘立酮、匹莫齐特、Z-舒必利和氟哌噻吨逆转DA诱导的cAMP蓄积抑制，其效力的等级顺序与逆转DA诱导的cAMP蓄积的效力的等级顺序相当。
The possible involvement of cAMP in mediating the dopaminergic inhibition of PRL release was studied in primary cultures of anterior pituitary cells from female rats and in lactotroph-enriched subpopulations. In the presence of the phosphodiesterase inhibitor isobutylmethylxanthine (IBMX), dopamine (DA) depressed cAMP accumulation in a concentrationdependent fashion between 1–100 nM. The effect was significant within less than 1 min and maximal after about 10 min. Inhibition of PRL release by DA occurred at similar concentrations and paralleled the time course of inhibition of cAMP levels. The DA agonist apomorphine also inhibited cAMP levels and PRL release at nanomolar concentrations and acted as a full agonist, whereas the a-adrenoceptor agonist phenylephrine had no effect at 1 μM. The DA receptor blockers spiperone, domperidone, pimozide, Z-sulpiride, and flupentixol reversed the DA-induced inhibition of cAMP accumulation with a rank order of potency comparable to that of reversing DA-induced inhibiti...
影响因子：
4.1
作者：
THORNER, MO;HACKETT, JT;MACLEOD, RM
通讯作者：
MACLEOD, RM
腺苷 3,5-单磷酸在多巴胺能抑制垂体前叶细胞催乳素释放中的作用。
影响因子：
4.8
作者：
Tam,SW;Dannies,PS
通讯作者：
Dannies,PS