Influences of mechanical barrier permeability on guided bone augmentation in the rat calvarium
Influences of mechanical barrier permeability on guided bone augmentation in the rat calvarium
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DOI：
10.2334/josnusd.17-0362
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发表时间：
2018-09-01
影响因子：
1.9
通讯作者：
Sato, Shuichi
中科院分区：
文献类型：
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作者：
Yamamoto, Takanobu;Hasuike, Akira;Sato, Shuichi
我们使用放射学和组织学分析来评价颅骨引导骨增量（GBA）大鼠模型中机械屏障渗透性的影响。暴露20只大鼠的颅骨，并将4种类型的塑料帽（闭合圆柱形塑料帽;无顶塑料帽;三孔塑料帽;和四孔塑料帽）中的一种随机放置在两侧。用显微计算机断层扫描（micro-CT）和组织学分析评价塑料帽中新生成的骨。显微CT体积分析和脱钙苏木精和伊红染色切片显示GBA屏障渗透性与获得的增强骨数量呈负相关。Masson三色染色显示，在新生成的骨组织中，三孔塑料帽中的胶原蛋白比具有更高渗透性或更封闭性屏障的塑料帽中的胶原蛋白更成熟。在通过穿透孔侵入软组织的标本中，骨增量受到抑制，屏障渗透性与增量骨的数量相关。总之，适度的屏障渗透性对于成熟的增强骨的发育是最佳的。
We used radiological and histological analyses to evaluate the effects of mechanical barrier permeability in a rat model of calvarial guided bone augmentation (GBA). The calvaria of 20 rats were exposed, and one of four types of plastic caps (an occlusive cylindrical plastic cap; a plastic cap with no top; a plastic cap with three holes; and a plastic cap with four holes) was randomly placed on both sides. Newly generated bone in the plastic caps was evaluated with micro-computed tomography (micro-CT) and histological analysis. Micro-CT volumetric analysis and decalcified hematoxylin and eosin-stained sections showed that GBA barrier permeability was inversely associated with the quantity of augmented bone obtained. Masson's trichrome staining showed that collagen in newly generated bony tissue was more mature in plastic caps with three holes than in those with more-permeable or more-occlusive barriers. Bone augmentation was inhibited in specimens exhibiting invasion of soft tissue through penetrating holes, and barrier permeability was associated with the quantity of augmented bone developed. In conclusion, moderate barrier permeability is optimal for development of mature augmented bone.