Nucleotide sequence and genome organization of a new proposed crinivirus, tetterwort vein chlorosis virus
Nucleotide sequence and genome organization of a new proposed crinivirus, tetterwort vein chlorosis virus
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DOI：
10.1007/s00705-015-2552-6
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发表时间：
2015-08
影响因子：
2.7
通讯作者：
F. Zhao;R. H. Yoo;Seungmo Lim;D. Igori;Su-Heon Lee;J. Moon
中科院分区：
文献类型：
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作者：
F. Zhao;R. H. Yoo;Seungmo Lim;D. Igori;Su-Heon Lee;J. Moon
来自韩国的粉葛脉褪绿病毒（TVCV）的基因组已经完全测序。其基因组结构与其他海百合病毒相似，具有几个新特征，表明TVCV是Closteroviridae科海百合病毒属的一个新种。RNA 1基因全长8467个核苷酸，至少有4个开放阅读框（ORF）。ORF1a编码具有预测的木瓜蛋白酶样蛋白酶、甲基转移酶和解旋酶活性的蛋白质。ORF1b编码一种假定的RNA依赖性RNA聚合酶，其显然通过+1核糖体移码表达。RNA2含有8113个核苷酸，编码至少9种蛋白质，与大多数海百合病毒RNA2相似。二分RNA基因组的3′非翻译区具有82.1%的核苷酸序列同一性。
The genome of tetterwort vein chlorosis virus (TVCV) from South Korea has been completely sequenced. Its genomic organization resembles those of other criniviruses, with several new features, indicating that TVCV is a member of a new species in the genusCrinivirus, familyClosteroviridae. RNA1 contains 8467 nucleotides, with at least four opening reading frames (ORFs). ORF1a encodes a protein with predicted papain-like protease, methyltransferase, and helicase activities. ORF1b encodes a putative RNA-dependent RNA polymerase that is apparently expressed through a +1 ribosomal frameshift. RNA2 contains 8113 nucleotides encoding at least nine proteins, similar to most crinivirus RNA2s. The 3′ untranslated regions of the bipartite RNA genome share 82.1 % nucleotide sequence identity.