DNA interstrand cross-link repair in the cell cycle -: A critical role for polymerase ζ in G1 phase
DNA interstrand cross-link repair in the cell cycle -: A critical role for polymerase ζ in G1 phase
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DOI：
10.4161/cc.5.10.2763
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发表时间：
2006-05-15
期刊：
影响因子：
4.3
通讯作者：
Sarkar, Sovan
中科院分区：
文献类型：
--
作者：
McHugh, Peter J.;Sarkar, Sovan
DNA链间交联（ICL）对细胞修复机制提出了严峻的挑战，但迄今为止ICL修复途径已被证明难以从遗传学角度进行剖析。现在看来，这部分是由于在选择ICL途径时高度的细胞周期阶段选择性。在这里，我们回顾最近的结果表明，聚合酶zeta，专门translesion在ICL修复在G（1）期酵母细胞中起着重要的作用，而PCNA的泛素修饰是其活性的关键调节因子。考虑到这种反应可以发生在S期的背景之外，这些结果意味着PCNA修饰在细胞周期中控制DNA修复途径中的更普遍的作用，这取决于所遇到的损伤或修复中间体的类型。
DNA interstrand cross-links (ICLs) present a formidable challenge to the cellular repair apparatus, but to date ICL repair pathways have proved difficult to dissect genetically. It now appears that this is partly the result of a high degree of cell cycle phase selectivity in the choice of ICL pathway employed. Here we review recent results showing that Polymerase zeta, specialized translesion plays an important role during ICL repair in G(1) phase yeast cells, and that PCNA modification by ubiquitin is a key regulator of its activity. Given that this reaction can occur outside the context of S-phase, these results imply a more general role for PCNA modification in the control of DNA repair pathways through the cell cycle, which is dependent on the type of damage or repair intermediate encountered.