Polystyrene nanoplastics alter virus replication in orange-spotted grouper (Epinephelus coioides) spleen and brain tissues and spleen cells
Polystyrene nanoplastics alter virus replication in orange-spotted grouper (Epinephelus coioides) spleen and brain tissues and spleen cells
复制标题
聚苯乙烯纳米塑料改变橙斑石斑鱼（Epinephelus coioides）脾脏、脑组织和脾细胞中的病毒复制
DOI：
10.1016/j.jhazmat.2021.125918
复制
发表时间：
2021-04-27
影响因子：
13.6
通讯作者：
Zhao, Huihong
中科院分区：
文献类型：
--
作者：
Wang, Qing;Duan, Xuzhuo;Zhao, Huihong
聚苯乙烯纳米塑料（PS-NP）已知会损害海洋生物的消化系统、肠道菌群、免疫系统和神经系统的功能。我们测试了 PS-NP 是否影响橙斑石斑鱼（Epinephelus coioides）的病毒感染。我们发现石斑鱼脾 (GS) 细胞在暴露浓度为 5、50 和 500 μg/mL 时吸收 PS-NP，并在 50 和 500 μg/mL 浓度下经历细胞毒性。暴露于50μg/mL PS-NPs后12小时，新加坡石斑鱼虹彩病毒（SGIV）和红斑石斑鱼神经坏死病毒（RGNNV）在入侵GS细胞后复制增加。幼鱼暴露于300和3000μg/L的PS-NPs 7天后，脾脏吸收PS-NPs并在脑组织中形成空泡。此外，PS-NP 暴露加速了脾脏中的 SGIV 复制和大脑中的 RGNNV 复制。 PS-NP暴露还降低了病毒侵入体外和体内前后的Toll样受体基因和干扰素相关基因的表达，从而降低了细胞和组织对病毒复制的抵抗力。这是首次报道PS-NPs对GS细胞以及脾脏和脑组织具有毒性作用，为评估PS-NPs对海水鱼类的影响提供了新的见解。
Polystyrene nanoplastics (PS-NPs) are known to impair the function of the digestive system, intestinal flora, immune system, and nervous system of marine organisms. We tested whether PS-NPs influence viral infection of orange-spotted grouper (Epinephelus coioides). We found that grouper spleen (GS) cells took up PS-NPs at exposure concentrations of 5, 50, and 500 mu g/mL and experienced cytotoxicity at 50 and 500 mu g/mL concentrations. At 12 h after exposure to 50 mu g/mL of PS-NPs, the replication of Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV) increased in GS cells after their invasion. Juvenile fish exposed to 300 and 3000 mu g/L of PS-NPs for 7 d showed PS-NPs uptake to the spleen and vacuole formation in brain tissue. Moreover, PS-NPs exposure accelerated SGIV replication in the spleen and RGNNV replication in the brain. PS-NP exposure also decreased the expression of toll-like receptor genes and interferon-related genes before and after virus invasion in vitro and in vivo, thus reducing the resistance of cells and tissues to viral replication. This is the first report that PS-NPs have toxic effects on GS cells and spleen and brain tissues, and it provides new insights into assessing the impact of PS-NPs on marine fish.