Detection of Epstein-Barr virus (EBV)-encoded microRNAs in plasma of patients with nasopharyngeal carcinoma
Detection of Epstein-Barr virus (EBV)-encoded microRNAs in plasma of patients with nasopharyngeal carcinoma
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DOI：
10.1002/hed.25544
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发表时间：
2019-03-01
影响因子：
2.9
通讯作者：
Chan, Jimmy Yu-Wai
中科院分区：
文献类型：
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作者：
Gao, Wei;Wong, Thian-Sze;Chan, Jimmy Yu-Wai
背景
鼻咽癌（NPC）由爱泼斯坦 - 巴尔病毒（EBV）潜伏感染，可表达40种EBV BART微小RNA（miRNA）。观察到这些miRNA在鼻咽癌检测中的诊断效能存在差异。在此，我们对这些miRNA的效能进行了综合评估。
方法
对从鼻咽癌患者和非癌症捐献者分离的血浆核酸进行定量聚合酶链反应。
结果
对于原发性鼻咽癌，BART2 - 5P、BART6 - 3P、BART7 - 3P、BART7 - 5P、BART9 - 5P、BART11 - 3P、BART17 - 5P和BART19 - 5P显著升高。对于复发性鼻咽癌，BART2 - 3P、BART2 - 5P、BART5 - 3P、BART5 - 5P、BART6 - 3P、BART8 - 3P、BART9 - 5P、BART17 - 5P、BART19 - 3P和BART20 - 3P的血浆水平显著升高。曲线下面积（AUC）分析表明，BART19 - 5P在识别血清学EBV DNA检测不到的鼻咽癌方面表现最佳。对于复发性鼻咽癌，BART8 - 3P和BART10 - 3P在识别EBV DNA检测不到的血浆中的癌症具有最高的AUC值。
结论
我们的数据支持在鼻咽癌和复发性鼻咽癌检测中使用循环EBV miRNA。
Background Nasopharyngeal carcinoma (NPC) latently infected by Epstein-Barr virus (EBV) expresses 40 EBV BART microRNAs (miRNAs). Difference in diagnostic efficacy of these miRNAs on NPC detection was observed. Here, we performed a comprehensive evaluation on the efficacy of these miRNAs. Methods Quantitative polymerase chain reaction was performed on plasma nucleic acid isolated from patients with NPC and noncancer donors. Results For primary NPC, BART2-5P, BART6-3P, BART7-3P, BART7-5P, BART9-5P, BART11-3P, BART17-5P, and BART19-5P were significantly elevated. For recurrent NPC, plasma levels of BART2-3P, BART2-5P, BART5-3P, BART5-5P, BART6-3P, BART8-3P, BART9-5P, BART17-5P, BART19-3P, and BART20-3P were significantly increased. Area under curve (AUC) analysis showed that BART19-5P had the best performance to identify NPC which was serologically EBV DNA undetectable. For recurrent NPC, BART8-3P and BART10-3P had highest AUC value for identifying cancer in EBV DNA undetectable plasma. Conclusion Our data supported the use of circulating EBV miRNAs in NPC and recurrent NPC detection.