Inactive allele-specific methylation and chromatin structure of the imprinted gene U2af1-rs1 on mouse chromosome 11.
Inactive allele-specific methylation and chromatin structure of the imprinted gene U2af1-rs1 on mouse chromosome 11.
复制标题
小鼠 11 号染色体上印记基因 U2af1-rs1 的非活性等位基因特异性甲基化和染色质结构。
DOI：
10.1006/geno.1996.0348
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发表时间：
1996
期刊：
影响因子：
4.4
通讯作者：
Hayashizaki,Y
中科院分区：
文献类型：
--
作者：
Shibata,H;Yoshino,K;Sunahara,S;Gondo,Y;Katsuki,M;Ueda,T;Kamiya,M;Muramatsu,M;Murakami,Y;Kalcheva,I;Plass,C;Chapman,VM;Hayashizaki,Y
定位于小鼠11号染色体的imprintedU 2af 1-rs 1基因主要由父系等位基因表达。我们检测了U2 af 1-rs 1基因座及其周围基因组序列的甲基化，以确定伴随母体等位基因沉默的序列修饰程度。HapII或HhaI位点的分析表明，沉默的母亲等位基因在一段超过10 kb的CpG序列中高度甲基化。通过比较，表达的父本等位基因从转录区上游的CpG岛到2kb未甲基化。对U2 af 1-rs 1假定启动子的DNaseI超敏性分析显示，仅在未甲基化的表达父系等位基因上存在开放的染色质构象。这些结果表明，覆盖该基因及其上游CpG岛的等位基因特异性超甲基化在U2 af 1-rs 1的母体等位基因抑制中起作用，这反映在DNaseI超敏感位点的染色质构象改变中。
The imprintedU2af1-rs1gene that maps to mouse chromosome 11 is predominately expressed from the paternal allele. We examined the methylation of genomic sequences in and around theU2af1-rs1locus to establish the extent of sequence modifications that accompanied the silencing of the maternal allele. The analysis ofHapII orHhaI sites showed that the silent maternal allele was hypermethylated in a block of CpG sequences that covered more than 10 kb. By comparison, the expressed paternal allele was unmethylated from a CpG island upstream of the transcribed region through 2 kb. An analysis of DNaseI hypersensitivity of a putative promoter ofU2af1-rs1showed an open chromatin conformation only on the unmethylated, expressed paternal allele. These results suggest that allele-specific hypermethylation covering the gene and its upstream CpG island plays a role in maternal allele repression ofU2af1-rs1,which is reflected in altered chromatin conformation of DNaseI hypersensitive sites.