Inositol-requiring protein 1α signaling pathway is activated in the temporal cortex of patients with mesial temporal lobe epilepsy
Inositol-requiring protein 1α signaling pathway is activated in the temporal cortex of patients with mesial temporal lobe epilepsy
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DOI：
10.1007/s10072-012-1008-y
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发表时间：
2013-03-01
影响因子：
3.3
通讯作者：
Zhao, Yong-Bo
中科院分区：
文献类型：
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作者：
Liu, Gong-Lu;Wang, Kai-Yan;Zhao, Yong-Bo
越来越多的证据表明，反复惊厥可诱发内质网应激。肌醇需求蛋白1α(IRE1α)是内质网应激中一个重要的促凋亡分子，但目前尚不清楚IRE1α介导的信号通路是否参与了人类颞叶癫痫的发病过程。本文应用免疫荧光和免疫印迹分析方法，研究了32例难治性内侧颞叶癫痫患者切除的新皮质中IRE1α介导的内质网应激促凋亡信号通路。我们的结果表明，慢性癫痫可诱导内质网应激，IRE1α介导的内质网应激细胞凋亡信号通路参与反复癫痫发作后的脑损伤，这可能为预防癫痫所致脑损伤提供新的治疗靶点。
Accumulating evidence suggests that repeated seizures could induce endoplasmic reticulum (ER) stress. Inositol-requiring protein 1 alpha (IRE1 alpha) is a vital pro-apoptotic molecule in ER stress, but it remains unclear whether the signaling pathway mediated by IRE1 alpha is involved in human temporal lobe epilepsy. In this report, we investigated IRE1 alpha-mediated ER stress pro-apoptotic signaling pathway in resected anterior temporal neocortex from 32 patients with intractable mesial temporal lobe epilepsy by immunofluorescence and western blot analysis. Our results indicate that chronic epilepsy induces ER stress, and IRE1 alpha-mediated ER stress apoptotic signaling pathway is involved in brain damage after repeated seizures, which may provide a new therapeutic target to prevent brain damage caused by epilepsy.