Localization of the different hepatitis C virus core gene products expressed in COS-1 cells.
Localization of the different hepatitis C virus core gene products expressed in COS-1 cells.
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COS-1 细胞中表达的不同丙型肝炎病毒核心基因产物的定位。
DOI：
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发表时间：
1998
影响因子：
1.6
通讯作者：
S. Tisminetzky
中科院分区：
文献类型：
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作者：
E. Buratti;F. Baralle;S. Tisminetzky
在这项研究中，我们研究了两种主要形式的丙型肝炎病毒核心蛋白：191个残基的未成熟蛋白（p21）和173个残基的蛋白水解裂解产物（p19）在转染的COS-1细胞中的亚细胞定位。在这项研究中，与以往的研究不同，我们已经能够分别区分p21和p19的定位。这是通过在p21完整编码序列中添加c端HSV标签来实现的，并利用了它随后在p19产品中丢失的事实。为了获得p21和p19的准确定位，我们使用小鼠抗hsv Tag MAb和人类抗核心MAb （B12.F8）进行双免疫荧光研究。结果表明p21总是定位在核膜周围。另一方面，p19在细胞质中有不同程度的扩散。这些体内实验结果强化了丙型肝炎病毒核心蛋白加工调控之间的联系，这可能有助于调节其多种生物学功能。
In this study we have investigated the subcellular localization in transfected COS-1 cells of the two major forms of the hepatitis C virus core protein: the immature protein of 191 residues (p21) and its proteolytically cleaved product of 173 residues (p19). In this study, and unlike previous investigations, we have been able to distinguish separately the localization of p21 from p19. This was achieved by the addition of a C-terminal HSV Tag to the p21 full coding sequence, and exploiting the fact that it is subsequently lost in the p19 product. In order to obtain an accurate localization of both p21 and p19 we used a mouse anti-HSV Tag MAb together with a human anti-core MAb (B12.F8) to perform double immunofluorescence studies. The results have shown that p21 is always localized around the nuclear envelope. On the other hand, p19 can be found diffused in the cytoplasm to different degrees. These in vivo results reinforce the proposed links between the regulated processing of the hepatitis C virus core protein and the possibility that this may contribute towards the regulation of its diverse biological functions.