Similarities and Differences between Colicin and Filamentous Phage Uptake by Bacterial Cells.
Similarities and Differences between Colicin and Filamentous Phage Uptake by Bacterial Cells.
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DOI：
10.1128/ecosalplus.esp-0030-2018
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Houot, Laetitia
中科院分区：
文献类型：
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作者：
Duche, Denis;Houot, Laetitia
革兰氏阴性菌已经进化出一种复杂的包膜，以适应和生存在广泛的生态位。这种物理屏障是防御有毒化合物和称为噬菌体的病毒颗粒的第一道防线。大肠杆菌素是由大肠杆菌和密切相关的细菌产生并对大肠杆菌和密切相关的细菌有毒的杀菌蛋白质家族。丝状病毒具有复杂的结构，由至少五种衣壳蛋白组装成一个长的线状颗粒，保护病毒DNA。尽管它们的大小和复杂性不同，但A组大肠杆菌素和丝状大肠杆菌素都寄生于其敏感宿主的多蛋白复合物中。它们首先与位于靶细菌表面的受体结合，然后特异性地招募Tol系统的组分穿过外膜并穿过周质。Tol系统被认为在细胞的生命周期中使用内膜的质子动力来维持外膜的完整性。本文介绍了A组大肠杆菌素和丝状噬菌体在被细菌宿主吸收过程中的顺序对接机制，特别关注通过与Tol系统的相互作用促进的易位步骤。
Gram-negative bacteria have evolved a complex envelope to adapt and survive in a broad range of ecological niches. This physical barrier is the first line of defense against noxious compounds and viral particles called bacteriophages. Colicins are a family of bactericidal proteins produced by and toxic to Escherichia coli and closely related bacteria. Filamentous phages have a complex structure, composed of at least five capsid proteins assembled in a long thread-shaped particle, that protects the viral DNA. Despite their difference in size and complexity, group A colicins and filamentous phages both parasitize multiprotein complexes of their sensitive host for entry. They first bind to a receptor located at the surface of the target bacteria before specifically recruiting components of the Tol system to cross the outer membrane and find their way through the periplasm. The Tol system is thought to use the proton motive force of the inner membrane to maintain outer membrane integrity during the life cycle of the cell. This review describes the sequential docking mechanisms of group A colicins and filamentous phages during their uptake by their bacterial host, with a specific focus on the translocation step, promoted by interactions with the Tol system.