The insecticide imidacloprid is a partial agonist of the nicotinic receptor of honeybee Kenyon cells
The insecticide imidacloprid is a partial agonist of the nicotinic receptor of honeybee Kenyon cells
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DOI：
10.1016/s0304-3940(01)02400-4
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发表时间：
2002-03-15
影响因子：
2.5
通讯作者：
Gauthier, M
中科院分区：
文献类型：
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作者：
Déglise, P;Grünewald, B;Gauthier, M
杀虫剂吡虫啉的主要作用靶点是昆虫脑内的神经烟碱乙酰胆碱受体（nAChRs）。研究了吡虫啉对培养的蜜蜂Kenyon细胞配体门控离子通道的影响。凯尼恩细胞形成了蘑菇体神经丸，它参与了更高阶的神经过程，如嗅觉学习。我们使用膜片钳技术通过烟碱和γ -氨基丁酸（GABA）受体测量了全细胞电流。吡虫啉的压力应用引发向内电流，这是不可逆的阻断α -班加罗毒素。吡虫啉是一种部分尼古丁激动剂，因为它只引起36%的乙酰胆碱诱导电流，并竞争性地阻断64%的乙酰胆碱诱导电流峰值。当吡虫啉共施时，gaba诱导的电流被部分阻断，这种阻断与nachr的激活无关。我们的研究结果表明，蜜蜂nAChR是吡虫啉的靶点，并且吡虫啉诱导了昆虫GABA受体的抑制。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
The main targets of the insecticide imidacloprid are neuronal nicotinic acetylcholine receptors (nAChRs) within the insect brain. We tested the effects of imidacloprid on ligand-gated ion channels of cultured Kenyon cells of the honeybee, Apis mellifera. Kenyon cells build up the mushroom body neuropils, which are involved in higher order neuronal processes such as olfactory learning. We measured whole-cell currents through nicotinic and gamma-aminobutyric acid (GABA) receptors using patch-clamp techniques. Pressure applications of imidacloprid elicited inward currents, which were irreversibly blocked by alpha-bungarotoxin. Imidacloprid was a partial nicotinic agonist, since it elicited only 36% of ACh-induced currents and competitively blocked 64% of the peak ACh-induced currents. GABA-induced currents were partially blocked when imidacloprid was coapplied and this block was independent upon activation of nAChRs. Our results identify the honeybee nAChR as a target of imidacloprid and an imidacloprid-induced inhibition of the insect GABA receptor. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.