Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that selectively labels developing thymus primordium.
Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that selectively labels developing thymus primordium.
复制标题
选择性标记发育中胸腺原基的 Tbx1-AmCyan1 转基因报告小鼠系的生成和表征。
DOI：
10.1007/s11248-012-9664-5
复制
发表时间：
2013
期刊：
影响因子：
3
通讯作者：
Kimura W et al.
中科院分区：
文献类型：
--
作者：
Zheng YW;Tsuchida T;Shimao T;Li B;Takebe T;Zhang RR;Sakurai Y;Ueno Y;Sekine K;Ishibashi N;Imajima M;Tanaka T;Taniguchi H;木村紘爾，大橋俊孝，中山 勝，小宮山高明，市村浩一，内藤一郎，浅野博昭，佃 和憲，内藤 稔，三好新一郎，大塚愛二，二宮善文．;Kimura W et al.
胸腺发育是一个复杂的过程，它包括高度动态的形态变化以及前肠内胚层来源的上皮细胞和神经沟来源的间充质细胞之间的相互组织相互作用。我们建立并鉴定了一只TBX1-AmCyan1报告转基因小鼠，以显示早期胚胎发育中的胸腺前体细胞。在转基因胚胎中，AmCyan1荧光在发育中的第3和第4咽囊的内胚层中特异地检测到，随后在胸腺上皮中检测到，直到胚胎14.5。根据AmCyan1荧光分离的表达AmCyan1的细胞表达内皮性、胸腺和甲状旁腺标志物，但不表达神经脊或内皮标志物，这些结果表明该转基因小鼠株可用于收集胸腺或甲状旁腺前体细胞或两者兼而有之。我们还发现，在胸腺发育中表现出缺陷的免疫器官，胸腺前体明显地被AmCyan1标记。综上所述，这些AmCyan1荧光转基因小鼠对于研究胸腺早期发育是有用的。
Thymus development is a complicated process that includes highly dynamic morphological changes and reciprocal tissue interactions between endoderm-derived epithelial cells of the anterior foregut and neural crest-derived mesenchymal cells. We generated and characterized a Tbx1-AmCyan1 reporter transgenic mouse to visualize thymus precursor cells during early embryonic development. In transgenic embryos, AmCyan1 fluorescence was specifically detected in the endoderm of the developing 3rd and 4th pharyngeal pouches and later in thymus epithelium until E14.5. Cells expressing AmCyan1 that were isolated based on AmCyan1 fluorescence expressed endodermal, thymic, and parathyroid markers, but they did not express neural crest or endothelial markers; these findings indicated that this transgenic mouse strain could be used to collect thymic or parathyroid precursor cells or both. We also showed that innudemice, which exhibit defects in thymus development, the thymus precursors were clearly labeled with AmCyan1. In summary, these AmCyan1-fluorescent transgenic mice are useful for investigating early thymus development.