Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.
Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.
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枯草芽孢杆菌孢子形成过程中参与区室化基因表达的新西格玛因子的鉴定。
DOI：
10.1101/gad.3.2.141
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发表时间：
1989
影响因子：
10.5
通讯作者：
Setlow,P
中科院分区：
文献类型：
--
作者：
Sun,DX;Stragier,P;Setlow,P
在枯草芽孢杆菌的孢子形成过程中，两个相同的基因组分离在两个隔室中，即前孢子和母细胞。这些基因组表达差异，一些基因如sspE仅在前孢子中开启。SspE的体外转录仅用从孢子形成细胞中提取的RNA聚合酶获得。分馏与这种酶和核心RNA聚合酶从营养细胞重建相关的因素产生的酶在体外准确转录sspE，并导致纯化的多肽与氨基末端序列的spoIIIG产品。失活的spoIIIG废除了表达的sspE和其他五个前孢子特异性基因，而合成的spoIIIG产品在营养cells迅速打开这些geneson.So，spoIIIG编码一个西格玛因子，西格玛G，它控制多个基因的表达在前孢子室。
During sporulation of Bacillus subtilis, two identical genomes segregate in two compartments, the forespore and mother cell. These genomes are expressed differentially, with some genes such as sspE turned on only in the forespore. In vitro transcription of sspE was obtained only with RNA polymerase extracted from sporulating cells. Fractionation of factors associated with this enzyme and reconstitution with core RNA polymerase from vegetative cells generated an enzyme accurately transcribing sspE in vitro and led to purification of a polypeptide with the amino-terminal sequence of the spoIIIG product. Inactivation of spoIIIG abolished expression of sspE and five other forespore-specific genes, whereas synthesis of the spoIIIG product in vegetative cells rapidly turned these genes on. Therefore, spoIIIG encodes a sigma-factor, sigma G, which controls the expression of multiple genes in the forespore compartment.