2 IMPROVED AND SIMPLIFIED METHODS FOR SPECTROPHOTOMETRIC DETERMINATION OF HYDROXYPROLINE
2 IMPROVED AND SIMPLIFIED METHODS FOR SPECTROPHOTOMETRIC DETERMINATION OF HYDROXYPROLINE
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DOI：
10.1021/ac60205a053
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发表时间：
1963-01-01
影响因子：
7.4
通讯作者：
LOXLEY, R
中科院分区：
文献类型：
--
作者：
BERGMAN, I;LOXLEY, R
本文在Stege-mann分光光度法的基础上，提出了一种测定羟脯氨酸的新方法。在试剂的稳定性和最终颜色方面取得了根本性的改进。在分析方法的各个阶段，颜色产率随条件的变化已降至最低，并且在比迄今报道的更宽的范围内与羟脯氨酸浓度无关。此外，还开发了一种不涉及恒温浴的方法，因为显色是在室温下过夜进行的，而不是在60 ℃下精确定时的时间内进行的。
A method, based cm that of Stege-mann but with a simplified procedure, has been developed for the spectrophotometric determination of hydroxyproline. Radical improvements have been achieved in the stability of the reagents and of the final color. The variation of the color yield with the conditions at various stages in the analytical procedure has been minimized, and been made independent of hydroxyproline concentration over a wider range than hitherto reported. In addition, a procedure has been developed which involves no thermostat bath, as the color development is carried out overnight at room tem-perature instead of in an accurately timed period at 60 C.