Ethanol regulates calcium channels in clonal neural cells.
Ethanol regulates calcium channels in clonal neural cells.
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DOI：
10.1073/pnas.83.16.6213
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发表时间：
1986-08
影响因子：
11.1
通讯作者：
Robert O. Messing;C. L. Carpenter;Ivan Diamond;David A. Greenberg
中科院分区：
文献类型：
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作者：
Robert O. Messing;C. L. Carpenter;Ivan Diamond;David A. Greenberg
在神经嵴来源的克隆细胞系PC12中，研究了乙醇对电压依赖性Ca通道功能的急性和长期影响。急性暴露于乙醇会产生浓度依赖性的去极化诱导的45Ca2+摄取减少，而长时间（2-10天）暴露会导致45Ca2+摄取和由二氢吡啶钙通道拮抗剂[3H]尼群地平标记的钙通道结合位点数量的增加。从培养物中提取乙醇后，摄取恢复到控制水平。这些发现表明，细胞对乙醇的适应可能涉及到二氢吡啶敏感、电压依赖的Ca通道的表达增强。
The acute and long-term effects of ethanol on voltage-dependent Ca channel function were studied in PC12, a clonal cell line of neural crest origin. Acute exposure to ethanol produced a concentration-dependent decrease in depolarization-evoked 45Ca2+ uptake, while prolonged (2-10 days) exposure led to a reciprocal increase in 45Ca2+ uptake and in the number of Ca-channel binding sites labeled by the dihydropyridine Ca-channel antagonist [3H]nitrendipine. Uptake was restored to control levels following withdrawal of ethanol from cultures. These findings indicate that cellular adaptation to ethanol may involve enhanced expression of dihydropyridine-sensitive, voltage-dependent Ca channels.