Amplified transduction of biomolecular interactions based on the use of nanomaterials.
Amplified transduction of biomolecular interactions based on the use of nanomaterials.
复制标题
基于纳米材料的使用放大生物分子相互作用的转导。
DOI：
10.1007/10_2007_074
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Wang,Joseph
中科院分区：
文献类型：
--
作者：
Wang,Joseph
实现非常高的灵敏度是生物测定中的主要目标， DNA杂交或蛋白质相互作用。本章回顾了纳米材料开发的进展 并讨论了不同的纳米材料为基础的生物放大策略。的出现 纳米技术的发展为高灵敏度的生物测定和新型生物传感器协议开辟了新的视野 利用电子、光学或微重力信号传导。功能化的抗体或核酸 与金属或半导体纳米颗粒一起已经被用作用于生物检测的放大标签， 蛋白质和DNA。不同的基于纳米材料的扩增平台和扩增的耦合 这一过程极大地增强了分析读数的强度，并导致超灵敏的生物测定。 新的纳米材料传感协议的显着灵敏度开辟了检测的可能性 疾病标志物、生物威胁因子或感染因子不能通过常规方法测量。
The achievement of very high sensitivity is a major goal in biological assays such as the monitoring of DNA hybridization or protein interactions. This chapter reviews progress in the development of nanomaterials for amplified biosensing and discusses different nanomaterial-based bioamplification strategies. The emergence of nanotechnology is opening new horizons for highly sensitive bioassays and for novel biosensor protocols that employ electronic, optical, or microgravimetric signal transduction. Antibodies or nucleic acids functionalized with metal or semiconductor nanoparticles have been employed as amplifying tags for the biodetection of proteins and DNA. The coupling of different nanomaterial-based amplification platforms and amplification processes dramatically enhances the intensity of the analytical readout and leads to ultrasensitive bioassays. The remarkable sensitivity of the new nanomaterial-based sensing protocols opens up the possibility of detecting disease markers, biothreat agents, or infectious agents that cannot be measured by conventional methods.