Genome sequence of the progenitor of the wheat D genome Aegilops tauschii.
Genome sequence of the progenitor of the wheat D genome Aegilops tauschii.
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DOI：
10.1038/nature24486
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发表时间：
2017-11-23
期刊：
影响因子：
64.8
通讯作者：
Dvořák J
中科院分区：
文献类型：
--
作者：
Luo MC;Gu YQ;Puiu D;Wang H;Twardziok SO;Deal KR;Huo N;Zhu T;Wang L;Wang Y;McGuire PE;Liu S;Long H;Ramasamy RK;Rodriguez JC;Van SL;Yuan L;Wang Z;Xia Z;Xiao L;Anderson OD;Ouyang S;Liang Y;Zimin AV;Pertea G;Qi P;Bennetzen JL;Dai X;Dawson MW;Müller HG;Kugler K;Rivarola-Duarte L;Spannagl M;Mayer KFX;Lu FH;Bevan MW;Leroy P;Li P;You FM;Sun Q;Liu Z;Lyons E;Wicker T;Salzberg SL;Devos KM;Dvořák J
结合先进的测序和作图技术，产生了一个参考基因组的节节麦，小麦D基因组的祖先，提供了一个宝贵的资源，比较遗传研究。本文的在线版本（doi：10.1038/nature 24486）包含补充材料，可供授权用户使用。农作物基因组测序为作物改良和育种提供了有用的资源。Jan Dvoclák、Katrien Devos、Steven Salzberg及其同事报告了粗山羊草的参考基因组，粗山羊草是六倍体小麦D基因组的二倍体祖先。他们结合使用有序克隆基因组测序、全基因组鸟枪测序和BioNano光学基因组图谱来组装这个庞大且高度重复的基因组。这为小麦的比较基因组学研究提供了有用的资源。本文的在线版本（doi：10.1038/nature 24486）包含补充材料，可供授权用户使用。节节麦是六倍体小麦（Triticum aestivum，genomes AABBDD）D基因组的二倍体祖先，是重要的小麦遗传资源。Ae的大尺寸和高度重复的性质。tauschii基因组迄今为止还排除了参考质量基因组序列的开发。在这里，我们使用一系列先进的技术，包括有序克隆基因组测序，全基因组鸟枪测序，和BioNano光学基因组定位，以产生一个参考质量的基因组序列的Ae。节节花亚种strangulata登录号AL 8/78，其与小麦D基因组密切相关。我们表明，相比其他测序的植物基因组，包括一个更大的针叶树基因组，AE。tauschii基因组包含了前所未有数量的非常相似的重复序列。我们的基因组比较显示，Ae.节节麦基因组比其他已测序基因组具有更多的分散的重复基因，并且其染色体在结构上的进化比其他禾本科基因组快一个数量级。与其他禾本科植物基因组共线性的衰减与染色体上的重组率沿着。我们认为，大量非常相似的重复序列会导致重组中的频繁错误，并导致基因复制和染色体结构变化，从而推动基因组的快速进化。本文的在线版本（doi：10.1038/nature 24486）包含补充材料，可供授权用户使用。
A combination of advanced sequencing and mapping techniques is used to produce a reference genome of Aegilops tauschii, progenitor of the wheat D genome, providing a valuable resource for comparative genetic studies. The online version of this article (doi:10.1038/nature24486) contains supplementary material, which is available to authorized users. Sequencing the genomes of crops plants provides useful resources for crop improvement and breeding. Jan Dvořák, Katrien Devos, Steven Salzberg and colleagues report a reference genome for Aegilops tauschii, the diploid progenitor of the D genome of hexaploid wheat. They use a combination of ordered-clone genome sequencing, whole-genome shotgun sequencing and BioNano optical genome mapping to assemble this large and highly repetitive genome. This provides a useful resource for comparative genomics studies of wheat. The online version of this article (doi:10.1038/nature24486) contains supplementary material, which is available to authorized users. Aegilops tauschii is the diploid progenitor of the D genome of hexaploid wheat (Triticum aestivum, genomes AABBDD) and an important genetic resource for wheat. The large size and highly repetitive nature of the Ae. tauschii genome has until now precluded the development of a reference-quality genome sequence. Here we use an array of advanced technologies, including ordered-clone genome sequencing, whole-genome shotgun sequencing, and BioNano optical genome mapping, to generate a reference-quality genome sequence for Ae. tauschii ssp. strangulata accession AL8/78, which is closely related to the wheat D genome. We show that compared to other sequenced plant genomes, including a much larger conifer genome, the Ae. tauschii genome contains unprecedented amounts of very similar repeated sequences. Our genome comparisons reveal that the Ae. tauschii genome has a greater number of dispersed duplicated genes than other sequenced genomes and its chromosomes have been structurally evolving an order of magnitude faster than those of other grass genomes. The decay of colinearity with other grass genomes correlates with recombination rates along chromosomes. We propose that the vast amounts of very similar repeated sequences cause frequent errors in recombination and lead to gene duplications and structural chromosome changes that drive fast genome evolution. The online version of this article (doi:10.1038/nature24486) contains supplementary material, which is available to authorized users.
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DOI：
10.1007/978-0-387-77489-3_23
发表时间：
2009-01-01
期刊：
GENETICS AND GENOMICS OF THE TRITICEAE
影响因子：
--
作者：
Dvorak, Jan
通讯作者：
Dvorak, Jan
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
3.3
作者：
Dvorak, J;Yang, ZL;Luo, MC
通讯作者：
Luo, MC
影响因子：
14.9
作者：
Kelley, JM;Field, CE;Adams, MD
通讯作者：
Adams, MD
影响因子：
9.2
作者：
Cao H;Hastie AR;Cao D;Lam ET;Sun Y;Huang H;Liu X;Lin L;Andrews W;Chan S;Huang S;Tong X;Requa M;Anantharaman T;Krogh A;Yang H;Cao H;Xu X
通讯作者：
Xu X