Physiological response of the symbiotic dinoflagellate Pelagodinium beii to ultraviolet radiation: synthesis and accumulation of mycosporine-like amino acids
Physiological response of the symbiotic dinoflagellate Pelagodinium beii to ultraviolet radiation: synthesis and accumulation of mycosporine-like amino acids
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共生甲藻 Pelagodinium beii 对紫外线辐射的生理反应：类菌孢素氨基酸的合成和积累
DOI：
10.1007/s13199-022-00839-y
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发表时间：
2022
期刊：
影响因子：
2.5
通讯作者：
Satoka SHIGEOKA and Makoto HIRAYAMA
中科院分区：
文献类型：
--
作者：
Aya Yamamoto HOSHIHARA;Tetsuichi FUJIKI;Satoka SHIGEOKA and Makoto HIRAYAMA
我们研究了共生甲藻Pelagoddium béiito对紫外线(UV)辐射的生理响应，重点研究了UV吸收物质(类霉孢素氨基酸，MAAs)的合成和积累。在培养实验中，我们用P。贝里是从球囊有孔虫中分离出来的，这种有孔虫无处不在地生活在营养不良的海洋表层水域。塞普。紫外光照射后12小时内，紫外光照射后12小时内，细胞内主要产生紫草碱、对苯二甲酸和霉菌素-甘氨酸作为MAAs，并与UVB照射成比例。单独UVA照射后，MAAs无明显积聚。我们观察到，即使在低UVB照射下，生长速度、细胞叶绿素含量和光合作用活性在2天内也会下降。我们的实验室实验表明，P。Béi i可以合成MAAs作为对UVB暴露的响应，但这种细胞生长和光合作用功能可能会受到自然环境表层水中UVB水平的抑制。我们的结果表明，自由生活的P。Béi i无法在Holobion OFP居住的表面水域中生存。BéII及其寄主球果球果，即使营养限制的胁迫得到了缓解。
We investigated the physiological response of the symbiotic dinoflagellatePelagodinium béiito exposure to ultraviolet (UV) radiation, focusing on synthesis and accumulation of UV-absorbing substances (mycosporine-like amino acids, MAAs). For the culture experiments, we usedP. béiiisolated from the foraminiferGlobigerinoides sacculifer, which ubiquitously inhabits the surface waters of oligotrophic oceans. TheP. béiiproduced mainly shinorine, palythenic acid, and mycosporine-glycine as MAAs, and they accumulated MAAs within cells in proportion to UVB exposure within 12 h after UV irradiation. There was no marked accumulation of MAAs with irradiation by only UVA. We observed decreases in growth rate, cellular chlorophyllacontent, and photosynthetic activity within 2 days, even under low UVB exposure. Our laboratory experiments showed thatP. béiicould synthesize MAAs in response to UVB exposure, but that cell growth and photosynthetic function could be inhibited by UVB levels in the surface waters of the natural environment. Our results suggested that free-livingP. béiicould not survive in the surface waters inhabited by the holobiont ofP. béiiand its hostGlobigerinoides sacculifer, even if the stress from nutrient limitation was reduced.